{
  "abstract": "Background/purpose\n\nLow-level Er:YAG laser irradiation has been reported to promote angiogenesis-related endothelial responses and enhance periodontal tissue healing. However, its effects on angiogenesis under hyperglycemic conditions have not been investigated. This study aimed to investigate whether low-level Er:YAG laser irradiation enhances the angiogenic response under hyperglycemic conditions.Materials and methods\n\nHuman umbilical vein endothelial cells (HUVECs) were cultured in control-glucose (CG, 5.5 mM) or high-glucose (HG, 25 mM) medium and subjected to low-level laser irradiation (LLLI) using an Er:YAG laser at 10 Hz and 30 mJ/pulse for 30 s under two pulse-widths. Angiogenesis-related cellular functions were evaluated by assessing cell proliferation, migration, tube formation, and mRNA expression. Intracellular reactive oxygen species (ROS) levels were measured.Results\n\nAlthough cell proliferation and migration significantly decreased under HG conditions (P < 0.01, P < 0.001), both were significantly restored after LLLI, reaching levels comparable to or higher than those in the HG control group. Under HG conditions, tube formation decreased; however, after LLLI, tube formation recovered, with significantly increased numbers of nodes, junctions, and segments, compared to those in the CG control group (P < 0.05). mRNA expression was not affected by HG medium, but vascular endothelial growth factor (VEGF ) and angiopoietin 1 (ANGPT1) expression significantly increased following LLLI (P < 0.05). ROS levels were significantly increased in the HG control group (P < 0.001), whereas the laser-treated groups showed significantly reduced ROS levels under HG conditions (P < 0.01).Conclusion\n\nLow-level Er:YAG laser irradiation improved angiogenesis-related cellular functions under hyperglycemic conditions.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Periodontology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo), Tokyo, Japan.",
    "Department of General Dentistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo), Tokyo, Japan.",
    "Department of Oral Biomedical Sciences, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo (Science Tokyo), Tokyo, Japan.",
    "School of Dentistry, Chung Shan Medical University (CSMU), Taichung, Taiwan.",
    "Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Bruna Akinaga Moreira"
    },
    {
      "affs": [
        1
      ],
      "name": "Koji Mizutani"
    },
    {
      "affs": [
        0
      ],
      "name": "Natsumi Saito"
    },
    {
      "affs": [
        0
      ],
      "name": "Lu Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Shu Takemura"
    },
    {
      "affs": [
        1
      ],
      "name": "Keita Nakagawa"
    },
    {
      "affs": [
        2
      ],
      "name": "Yujin Ohsugi"
    },
    {
      "affs": [
        0,
        3,
        4
      ],
      "name": "Taichen Lin"
    },
    {
      "affs": [
        0
      ],
      "name": "Takanori Iwata"
    },
    {
      "affs": [
        0
      ],
      "name": "Akira Aoki"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4rf7xkcakaap1.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jds.2025.07.3585",
  "fulltext_url": "https://europepmc.org/articles/PMC13689662",
  "id": "4rf7xkcakaap1",
  "issns": [
    "1991-7902",
    "2213-8862"
  ],
  "landing_url": "https://europepmc.org/article/MED/42923741",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2741|Laser Applications in Dentistry and Medicine"
    ]
  ],
  "openalex": "W7223566888",
  "pdf_url": "https://europepmc.org/articles/PMC13689662?pdf=render",
  "pmcid": "PMC13689662",
  "pmid": "42923741",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4rf7xkcakaap1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42923741",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42923741"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Laser Applications in Dentistry and Medicine",
      "subfield": "imaging"
    }
  ],
  "title": "Effects of low-level Er:YAG laser irradiation on endothelial angiogenic responses in diabetes: An in vitro study.",
  "title_text": "Effects of low-level Er:YAG laser irradiation on endothelial angiogenic responses in diabetes: An in vitro study.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/4rf7xkcakaap1",
  "venue": "Journal of dental sciences",
  "versions": []
}