{
  "abstract": "A Biomolecular condensates, formed through liquid–liquid phase separation (LLPS), play a crucial role in organizing various cellular functions. Materials that can regulate condensate dynamics without genetic modification could provide new tools for controlling biomolecular organization. In this study, we explored butterfly-shaped gold nanoparticles (GNBs) as regulators of the phase behavior of maltose-binding protein-tagged fused in sarcoma (MBP–FUS), a model protein containing both folded and intrinsically disordered regions. We established a phase diagram to identify the conditions near the boundary between homogeneous and phase-separated states. Under these conditions, GNBs were found to promote the formation of spherical MBP–FUS condensates, while spherical gold nanoparticles and gold nanorods did not have a similar effect. Additionally, irradiation at 660 nm reduced both the size and abundance of the GNB-associated condensates, consistent with a photothermal response. These findings show that GNBs can influence MBP–FUS condensation near a phase boundary and suggest that nanoparticle geometry plays a significant role in this behavior. This study lays the groundwork for developing externally controlled, gene-modification-free methods for regulating biomolecular condensates.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "Tomohiro Nobeyama",
      "orcid": "0000-0001-6127-7861"
    },
    {
      "name": "Keiji Kitamura"
    },
    {
      "name": "Ryo Kitahara"
    },
    {
      "name": "Ganesh N Pandian"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4rgh7pr255j1x.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15009684/v1",
  "fulltext_url": null,
  "id": "4rgh7pr255j1x",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009684/v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ]
  ],
  "openalex": "W7214929702",
  "pdf_url": "https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15009684/v1",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-30",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/4rgh7pr255j1x.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:21Z",
      "native_id": "10.26434/chemrxiv.15009684",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009684/v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA Research and Splicing",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Regulation of Fused in Sarcoma Condensate Formation and Dissolution Using Butterfly-Shaped Gold Nanoparticles",
  "title_text": "Regulation of Fused in Sarcoma Condensate Formation and Dissolution Using Butterfly-Shaped Gold Nanoparticles",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:21Z",
  "url": "https://research.draconitic.com/paper/4rgh7pr255j1x",
  "venue": "ChemRxiv",
  "versions": []
}