{
  "abstract": "Ethyl hydroxyethyl cellulose (EHEC) is a promising sustainable biomaterial, but its high hydrophilicity limits its use in moisture-resistant applications. Conventional cross-linking to improve aqueous resistance often requires toxic reagents, organic solvents, or high temperatures. Here, we developed a green cross-linking method by enzymatically oxidizing EHEC with laccase/TEMPO (2,2,6,6-tetramethylpiperidin-1-oxyl), followed by UV-induced cross-linking with chitosan at room temperature, yielding a robust macromolecular network. The optimal conditions (4 h oxidation, 50 wt % chitosan, 24 h cross-linking) yielded prolonged aqueous-stable films that maintained structural stability for 3–5 months in aqueous media, depending on pH. Compared to purified EHEC, the biomaterial exhibited enhanced thermal behavior, a 44% increase in elastic modulus (775.6 ± 172.8 MPa), reduced hydrophilicity (75.2 ± 0.7° contact angle, compared to 56.5 ± 0.6° from the original EHEC film), and no release of toxic compounds in cytotoxic assays with L929 fibroblast cells. This methodology is tunable and efficiently produces stable, sustainable EHEC-based films, demonstrating significant promise for advanced aqueous applications and active-ingredient encapsulation.",
  "abstract_shown": true,
  "affiliations": [
    "Universidade Federal de Santa Catarina , , Rua Marechal Rondon, 880 - Salto do Norte , , ,",
    "Centro Tecnológico , , Córrego Grande , , ,",
    "Universidade Federal de Santa Catarina , , Rua Roberto Sampaio Gonzaga, s/n - Trindade , , ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Beatriz Fernanda da Silva"
    },
    {
      "affs": [
        0
      ],
      "name": "Lidiane Meier"
    },
    {
      "affs": [
        0
      ],
      "name": "Ismael Casagrande Bellettini",
      "orcid": "0000-0002-9293-6492"
    },
    {
      "affs": [
        1
      ],
      "name": "Karina Cesca"
    },
    {
      "affs": [
        1
      ],
      "name": "Ana Paula Serafini Immich",
      "orcid": "0000-0001-6927-8041"
    },
    {
      "affs": [
        2
      ],
      "name": "Patrícia Bulegon Brondani",
      "orcid": "0000-0003-4786-8627"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4tf2kdpqe5ptr.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c08601",
  "fulltext_url": null,
  "id": "4tf2kdpqe5ptr",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c08601/5454045/Enzymatic-Bio-Oxidation-of-Ethyl-Hydroxyethyl",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2502|Advanced Cellulose Research Studies"
    ]
  ],
  "openalex": "W7223019593",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c08601",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-20",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/4tf2kdpqe5ptr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c08601",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c08601/5454045/Enzymatic-Bio-Oxidation-of-Ethyl-Hydroxyethyl"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2502|Advanced Cellulose Research Studies",
      "subfield": "materials-polymers"
    }
  ],
  "title": "Enzymatic Bio-Oxidation of Ethyl Hydroxyethyl Cellulose and UV-Assisted Cross-Linking with Chitosan for Enhanced Aqueous Stability in Biomaterial Production",
  "title_text": "Enzymatic Bio-Oxidation of Ethyl Hydroxyethyl Cellulose and UV-Assisted Cross-Linking with Chitosan for Enhanced Aqueous Stability in Biomaterial Production",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:39Z",
  "url": "https://research.draconitic.com/paper/4tf2kdpqe5ptr",
  "venue": "ACS Omega",
  "versions": []
}