{
  "abstract": "Introduction\n\nObese patients suffer from cardiovascular, metabolic and gastrointestinal disorders, including constipation, proposed to result from high fat diet (HFD)-induced enteric neuron loss. However, these studies used high-fat purified diets, containing reduced amounts of fiber compared to non-purified diets, known to alter gut physiology and microbiome composition. Therefore, we evaluated the effect of purified HFD-induced obesity on enteric neuron density and gastrointestinal transit compared to its proper control diet, that is, a purified control diet.Methods\n\nWild type mice were fed a standard chow diet, purified low fat diet (LFD) or HFD. Colonic transit was measured via glass bead expulsion following 12, 18, and 24 weeks of diet. Colonic muscularis externa was collected for immunofluorescent stainings of enteric neurons.Results\n\nStarting from 18 weeks of HFD, a decline in enteric neuron number was observed compared to LFD. In addition, delayed colonic transit was observed in HFD-fed mice when compared to LFD-fed animals after 18 weeks of exposure.Conclusions\n\nOur study demonstrates that prolonged exposure to a HFD results in a reduced enteric neuron density and delayed colonic transit, and underscores the need to use a proper control diet when evaluating the impact of a HFD on gut physiology.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory for Intestinal Neuro-Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases, Metabolism and Ageing, KU Leuven, Leuven, Belgium.",
    "Developmental Biology of the Immune System, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Marta Chavero-Pieres",
      "orcid": "0000-0003-1116-8397"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Maria Francesca Viola",
      "orcid": "0000-0003-0660-9506"
    },
    {
      "affs": [
        0
      ],
      "name": "Zheng Wang",
      "orcid": "0009-0007-5913-3990"
    },
    {
      "affs": [
        0
      ],
      "name": "Marcello Delfini",
      "orcid": "0000-0003-0342-5445"
    },
    {
      "affs": [
        0
      ],
      "name": "Iris Appeltans"
    },
    {
      "affs": [
        0
      ],
      "name": "Naomi Fabre",
      "orcid": "0000-0001-9316-3869"
    },
    {
      "affs": [
        0
      ],
      "name": "Guy Boeckxstaens",
      "orcid": "0000-0001-8267-5797"
    },
    {
      "affs": [
        0
      ],
      "name": "Nathalie Stakenborg",
      "orcid": "0000-0002-6229-0045"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4tga7cac04sxv.bib",
  "cited_by": 0,
  "doi": "10.1111/nmo.70470",
  "fulltext_url": "https://europepmc.org/articles/PMC13678409",
  "id": "4tga7cac04sxv",
  "issns": [
    "1350-1925",
    "1365-2982"
  ],
  "landing_url": "https://europepmc.org/article/MED/42894465",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2715|Gastrointestinal motility and disorders"
    ]
  ],
  "openalex": "W7222925819",
  "pdf_url": "https://europepmc.org/articles/PMC13678409?pdf=render",
  "pmcid": "PMC13678409",
  "pmid": "42894465",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4tga7cac04sxv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:37Z",
      "native_id": "MED:42894465",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42894465"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2715|Gastrointestinal motility and disorders",
      "subfield": "general"
    }
  ],
  "title": "High Fat Diet Alters Gastrointestinal Function and Results in Reduced Enteric Neuron Density.",
  "title_text": "High Fat Diet Alters Gastrointestinal Function and Results in Reduced Enteric Neuron Density.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:37Z",
  "url": "https://research.draconitic.com/paper/4tga7cac04sxv",
  "venue": "Neurogastroenterology and motility",
  "versions": []
}