{
  "abstract": "Co-expression and cross-regulation of the four TCF/LEFs render their redundant and unique functions ambiguous. Here, we describe quadruple-knockout (QKO) mouse ESCs lacking all full-length TCF/LEFs and cell lines rescued with TCF7 or TCF7L1. QKO cells self-renew, despite gene expression patterns that differ significantly from WT, and display delayed, neurectoderm-biased, embryoid body (EB) differentiation. QKO EBs have no contracting cardiomyocytes and differentiate poorly into mesendoderm but readily generate neuronal cells. QKO cells and TCF7L1-rescued cells cannot efficiently activate TCF reporters, whereas TCF7-rescued cells exhibit significant reporter responsiveness. Surprisingly, despite dramatically different transactivation capacities, re-expression of TCF7L1 or TCF7 in QKO cells restores their tri-lineage differentiation ability, with similar lineage marker expression patterns and beating cardiomyocyte frequencies observed in EBs. Both factors also similarly affect the transcriptome of QKO cells. Our data reveal that a single TCF, regardless of its activation capacity, is sufficient for effective trilineage differentiation of ESCs.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Steven Moreira"
    },
    {
      "name": "Enio Polena"
    },
    {
      "name": "Victor Gordon"
    },
    {
      "name": "Solen Abdulla"
    },
    {
      "name": "Sujeivan Mahendram"
    },
    {
      "name": "Jiayi Cao"
    },
    {
      "name": "Alexandre Blais"
    },
    {
      "name": "Geoffrey Wood"
    },
    {
      "name": "Anna Dvorkin-Gheva"
    },
    {
      "name": "Bradley Doble"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4tt4t1yebnhqx.bib",
  "cited_by": 40,
  "doi": "10.1016/j.celrep.2017.08.043",
  "fulltext_url": null,
  "id": "4tt4t1yebnhqx",
  "issns": [
    "2211-1247",
    "2211-1247"
  ],
  "landing_url": "https://hal.science/hal-05794900v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Pluripotent Stem Cells Research"
    ]
  ],
  "openalex": "W2752417195",
  "pdf_url": "http://www.cell.com/article/S2211124717311506/pdf",
  "pmcid": null,
  "pmid": "28877475",
  "published": "2026-10-12",
  "publisher": "Elsevier Inc",
  "ris": "https://research.draconitic.com/paper/4tt4t1yebnhqx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:54Z",
      "native_id": "hal-05794900",
      "source": "hal",
      "url": "https://hal.science/hal-05794900v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Pluripotent Stem Cells Research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A Single TCF Transcription Factor, Regardless of Its Activation Capacity, Is Sufficient for Effective Trilineage Differentiation of ESCs",
  "title_text": "A Single TCF Transcription Factor, Regardless of Its Activation Capacity, Is Sufficient for Effective Trilineage Differentiation of ESCs",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:54Z",
  "url": "https://research.draconitic.com/paper/4tt4t1yebnhqx",
  "venue": "Cell Reports",
  "versions": []
}