{
  "abstract": "Objectives\n\nChromosomal aneuploidy arises from errors in chromosome segregation during cell division, and embryonic aneuploidy is a major cause of implantation failure and miscarriage, which is primarily attributed to maternal meiotic errors. However, recent data accumulated from preimplantation genetic testing have suggested that chromosome segregation errors may also frequently occur during mitotic divisions in early embryos. One proposed factor for these errors is that the cell size in early embryos is larger than that of somatic cells after cleavage, but the exact mechanisms remain unclear.Methods\n\nTo determine the effect of cell size on the incidence of aneuploidy, we established a cultured cell model in which the cell size was artificially enlarged. This model harbored a chromosome carrying the GFP gene, and disappearance of GFP fluorescence served as a quantitative marker for aneuploidy events. By overexpressing genes that increase cell size, we measured the cell size and frequency of aneuploidy using flow cytometry.Results\n\nWe observed a significant increase (p=3.67×10⁻⁹) in the incidence of aneuploidy in large cells, which supports the hypothesis that a large cell size characteristic of early embryos contributes to the occurrence of chromosomal segregation errors.Conclusions\n\nOur finding may partly explain the high prevalence of aneuploid mosaicism in human preimplantation embryos.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Molecular Genetics, Center for Medical Science, Fujita Health University, Toyoake, Aichi, Japan.",
    "Office of Research Administration, Fujita Health University, Toyoake, Aichi, Japan.",
    "Department of Biochemistry, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.",
    "Biomedical Research Support Center, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Nagasaki, Japan.",
    "Department of Biomedical Data Science, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan.",
    "Laboratory of Data Management and Analysis, Open Facility Center, Fujita Health University, Toyoake, Aichi, Japan.",
    "Department of Obstetrics and Gynecology, Kansai Medical University, Hirakata, Osaka, Japan.",
    "Clinical Genetics Center, Kansai Medical University Hospital, Hirakata, Osaka, Japan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yoshimi Kato"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Makiko Tsutsumi"
    },
    {
      "affs": [
        2
      ],
      "name": "Takuya Abe"
    },
    {
      "affs": [
        3
      ],
      "name": "Kazuo Yamamoto"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Junichiro Yoshimoto"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Takayuki Kannon"
    },
    {
      "affs": [
        0,
        6,
        7
      ],
      "name": "Hiroki Kurahashi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4tz5wz31tq4c1.bib",
  "cited_by": 0,
  "doi": "10.20407/fmj.2026-004",
  "fulltext_url": "https://europepmc.org/articles/PMC13724193",
  "id": "4tz5wz31tq4c1",
  "issns": [
    "2189-7247",
    "2189-7255"
  ],
  "landing_url": "https://europepmc.org/article/MED/42968868",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "1307|Microtubule and mitosis dynamics"
    ]
  ],
  "openalex": "W7225111653",
  "pdf_url": "https://europepmc.org/articles/PMC13724193?pdf=render",
  "pmcid": "PMC13724193",
  "pmid": "42968868",
  "published": "2026-09-25",
  "publisher": "Fujita Medical Society",
  "ris": "https://research.draconitic.com/paper/4tz5wz31tq4c1.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T15:20:53Z",
      "native_id": "MED:42968868",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42968868"
    },
    {
      "first_seen": "2026-11-04T12:22:41Z",
      "native_id": "PMC13724193",
      "source": "pmc",
      "url": "https://doi.org/10.20407/fmj.2026-004"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Microtubule and mitosis dynamics",
      "subfield": "cell-biology"
    }
  ],
  "title": "Effects of cell size on incidence of chromosome aneuploidy: Analysis using a cultured cell model.",
  "title_text": "Effects of cell size on incidence of chromosome aneuploidy: Analysis using a cultured cell model.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:52Z",
  "url": "https://research.draconitic.com/paper/4tz5wz31tq4c1",
  "venue": "Fujita medical journal",
  "versions": []
}