{
  "abstract": "Corneal stromal bioprinting requires bioinks that combine optical transparency, structural stability, printability, and a biologically instructive microenvironment capable of maintaining keratocyte quiescence. Here, we report the development of GAME, a composite bioink integrating gelatin methacryloyl (GelMA) with murine amniotic membrane extract (AME), and comprehensively characterize its biochemical composition, physicochemical properties, bioprintability, and ability to support human induced pluripotent stem cell-derived corneal stromal keratocyte-like cells (hiPSC-CSKs). Quantitative proteomic analysis demonstrated that AME possesses a conserved proteome enriched in collagens, small leucine-rich proteoglycans, fibronectin, nidogens, and growth factor-associated regulatory proteins, with compositional consistency across independently prepared batches. Incorporation of AME into GelMA generated a transparent hydrogel with an interconnected porous microarchitecture, sustained structural stability, and elastic-dominant viscoelastic behavior within the range reported to support corneal stromal cell function. The GAME bioink also tolerated surgical suturing and enabled reproducible extrusion bioprinting of corneal-scale constructs with high printability and geometric fidelity following photocrosslinking. Bioprinted hiPSC-CSKs embedded in GAME exhibited high viability throughout 21 days of culture while maintaining expression of the keratocyte-associated markers keratocan, ALDH3A1, and CD34, with minimal α-smooth muscle actin (α-SMA) expression, indicating preservation of a quiescent, non-fibrotic stromal phenotype after bioprinting. These findings establish GAME as a compositionally defined, mechanically robust, and cytocompatible bioink for corneal stromal tissue engineering. More broadly, this work demonstrates that proteomic characterization of biologically derived supplements provides a rational framework for the development of reproducible and biologically instructive bioinks for corneal tissue engineering.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "Reem Al Monla"
    },
    {
      "name": "Manon Descieux"
    },
    {
      "name": "Morgane Benard"
    },
    {
      "name": "Melissa Girard"
    },
    {
      "name": "Laura Fichter"
    },
    {
      "name": "Sonia Garcia Lorens"
    },
    {
      "name": "Jerome Vialaret"
    },
    {
      "name": "Christophe Hirtz"
    },
    {
      "name": "Christopher Leon-Valdivieso"
    },
    {
      "name": "Vincent Daien"
    },
    {
      "name": "Marie Rose Rovere"
    },
    {
      "name": "Catherine Moali"
    },
    {
      "name": "Claire Bardet"
    },
    {
      "name": "Frederic Michon"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4vk370y4mxar9.bib",
  "cited_by": 0,
  "doi": "10.1016/j.bprint.2026.e00496",
  "fulltext_url": null,
  "id": "4vk370y4mxar9",
  "issns": [
    "2405-8866"
  ],
  "landing_url": "https://hal.science/hal-05762017v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2204|3D Printing in Biomedical Research"
    ]
  ],
  "openalex": "W7171406710",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-24",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/4vk370y4mxar9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:40Z",
      "native_id": "hal-05762017",
      "source": "hal",
      "url": "https://hal.science/hal-05762017v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    }
  ],
  "title": "Proteomics-supported amniotic membrane extract-supplemented GelMA bioink enables extrusion-based 3D bioprinting of hiPSC-derived corneal stromal constructs",
  "title_text": "Proteomics-supported amniotic membrane extract-supplemented GelMA bioink enables extrusion-based 3D bioprinting of hiPSC-derived corneal stromal constructs",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:40Z",
  "url": "https://research.draconitic.com/paper/4vk370y4mxar9",
  "venue": "Bioprinting",
  "versions": []
}