{
  "abstract": "Impaired skin repair is associated with insufficient fibroblast activity and dysregulated extracellular matrix turnover. Small extracellular vesicles (sEVs) derived from multipotent mesenchymal stromal cells (MMSCs) may provide a cell-free approach to modulate these processes; however, the effects of sEVs from genetically modified producer cells remain incompletely characterized. We evaluated sEV preparations obtained from native MMSCs and from MMSCs separately transfected with OCT4/SOX2, HIF-1alpha, or Klotho expression plasmids. A 1:1:1 mixture of sEV preparations from the three modified cell populations was examined as a combined treatment. Rat skin fibroblasts were exposed to the preparations for 24, 48, or 72 h. Fibroblast density and concentrations of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), collagen type I, and collagen type III were assessed. The combined sEV preparation produced the largest observed increase in fibroblast density, reaching 112.63 +/- 5.55 x 10^3 cells/cm2 at 72 h, compared with 40.60 +/- 1.12 x 10^3 cells/cm2 for native MMSC-derived sEVs and 37.10 +/- 3.01 x 10^3 cells/cm2 in the untreated control. At 24 h, MMP-1 and MMP-3 concentrations in the combined group were 140.0 +/- 4.77 ng/mL and 144.0 +/- 4.74 ng/mL, respectively, compared with 283.1 +/- 25.2 ng/mL and 208.1 +/- 39.2 ng/mL in controls. Collagen type III concentration increased to 1.95 +/- 0.25 ng/mL at 72 h versus 1.15 +/- 0.15 ng/mL in controls. These findings indicate that the combined sEV preparation was associated with enhanced fibroblast proliferation and altered extracellular matrix remodeling in vitro. Further work is required to define sEV composition, dose-response relationships, and in vivo efficacy and safety.",
  "abstract_shown": true,
  "affiliations": [
    "Ural State Medical University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "O. G. Makeev"
    },
    {
      "name": "A. V. Korotkov"
    },
    {
      "affs": [
        0
      ],
      "name": "A. A. Seliverstova"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4w0edyx3pykgj.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.17.752298",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752298v1.full",
  "id": "4w0edyx3pykgj",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752298v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7213534023",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752298v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-17",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4w0edyx3pykgj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:23:00Z",
      "native_id": "10.64898/2026.09.17.752298",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752298v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "Small extracellular vesicle-enriched preparations derived from genetically modified multipotent mesenchymal stromal cells promote fibroblast proliferation and extracellular matrix remodeling in vitro",
  "title_text": "Small extracellular vesicle-enriched preparations derived from genetically modified multipotent mesenchymal stromal cells promote fibroblast proliferation and extracellular matrix remodeling in vitro",
  "type": "preprint",
  "updated_at": "2026-10-30T12:23:00Z",
  "url": "https://research.draconitic.com/paper/4w0edyx3pykgj",
  "venue": "bioRxiv",
  "versions": []
}