{
  "abstract": "ABCG2 (BCRP/MXR) is an ATP-binding cassette transporter that plays a role in cellular resistance to various anticancer agents. While several non-synonymous single-nucleotide polymorphisms (SNPs) in the ABCG2 gene have been functionally characterized, the effects of many variants remain uncertain. This study examined the human ABCG2 SNP variant P269S (rs34678167, c.805C>T) in cellular resistance to mitoxantrone and SN-38 using a Flp-In-293 controlled expression system. Quantitative real-time PCR, whole-cell Western blotting, and cell-surface biotinylation analysis showed comparable ABCG2 mRNA and total protein levels and ABCG2 abundance in the biotinylated protein fraction between ABCG2(P269S)- and ABCG2(WT)-expressing cells. In MTT assays, ABCG2(P269S)-expressing cells exhibited significantly higher EC50 values than ABCG2(WT)-expressing cells, with mean values approximately 3.0- and 3.5-fold higher for mitoxantrone and SN-38, respectively. Consequently, P269S is associated with increased cellular resistance to mitoxantrone and SN-38 despite no detectable differences in total ABCG2 protein abundance or abundance in the biotinylated protein fraction. Collectively, these findings underscore the utility of the Flp-In-293 system for assessing variant-specific effects of ABCG2 on cellular drug responses within a controlled background. These results support future human pharmacogenomic research on rs34678167 to determine whether this variant contributes to inter-individual differences in responses to ABCG2 substrate drugs.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Bioscience and Biotechnology, Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan",
    "Department of Biological Chemistry, Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai 487-8501, Japan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shiori Sato"
    },
    {
      "affs": [
        1
      ],
      "name": "Megumi Tsukamoto"
    },
    {
      "affs": [
        0
      ],
      "name": "Kayo Ozawa"
    },
    {
      "affs": [
        0
      ],
      "name": "Ritsuko Imai"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hiroshi Nakagawa",
      "orcid": "0000-0002-9697-164X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4x9nzcbgkjhy1.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16101486",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702490/?tool=EBI",
  "id": "4x9nzcbgkjhy1",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/10/1486",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2730|Drug Transport and Resistance Mechanisms"
    ]
  ],
  "openalex": "W7221695336",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702490/pdf/?tool=EBI",
  "pmcid": "PMC13702490",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/4x9nzcbgkjhy1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:59Z",
      "native_id": "10.3390/biom16101486",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/10/1486"
    },
    {
      "first_seen": "2026-10-30T12:21:40Z",
      "native_id": "PMC:PMC13702490",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13702490"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|Drug Transport and Resistance Mechanisms",
      "subfield": "oncology"
    }
  ],
  "title": "A Human ABCG2 SNP Variant, P269S (rs34678167, c.805C>T), Increases Cellular Resistance to Mitoxantrone and SN-38 Without Detectable Differences in ABCG2 Abundance in Total and Biotinylated Protein Fractions",
  "title_text": "A Human ABCG2 SNP Variant, P269S (rs34678167, c.805C>T), Increases Cellular Resistance to Mitoxantrone and SN-38 Without Detectable Differences in ABCG2 Abundance in Total and Biotinylated Protein Fractions",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:40Z",
  "url": "https://research.draconitic.com/paper/4x9nzcbgkjhy1",
  "venue": "Biomolecules",
  "versions": []
}