{
  "abstract": "An antibacterial metabolite was isolated from the endophytic fungus Fusarium incarnatum JMB4 associated with Jatropha multifida L. by bioactivity-guided chromatography and recrystallization. The isolated compound was assigned as equisetin using UV–vis, FTIR, LC–MS/MS, and 1H NMR data. Anti-MRSA activity was evaluated by disk diffusion, with chloramphenicol and amoxicillin as antibiotic comparators. At 1% (w/v; 100 μg/disk), equisetin produced an inhibition zone of 23.94 ± 0.66 mm; the recorded comparator zones were 24.71 ± 0.68 mm for chloramphenicol (30 μg/disk) and 11.76 ± 0.66 mm for amoxicillin (25 μg/disk). The lowest tested equisetin concentration producing a measurable zone was 78.1 μg/mL (7.03 ± 0.25 mm). This diffusion end point does not represent a minimum inhibitory concentration (MIC), and the unequal disk loads preclude a direct comparison of intrinsic antibacterial potency. Exploratory docking against penicillin-binding protein 2a (PBP2a) yielded a score of −5.5041 kcal/mol and a predicted pose outside the canonical catalytic pocket. A 100 ns molecular dynamics simulation described the behavior of the modeled complex but did not establish PBP2a inhibition. These results document anti-MRSA activity of the isolated compound under the tested conditions; standardized MIC determination and further structural validation remain necessary.",
  "abstract_shown": true,
  "affiliations": [
    "National Cheng Kung University , , 1 University Road , ,",
    "Universitas Andalas , , Kampus Limau Manis , ,",
    "Akademi Farmasi Cendikia Farma Husada , Jl. Pulau Enggano–Tirtayasa No. 100, Sukabumi , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Herland Satriawan"
    },
    {
      "affs": [
        1
      ],
      "name": "Rachel Shammara Khalda"
    },
    {
      "affs": [
        0
      ],
      "name": "Hao-Ze Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Arif Setiawansyah",
      "orcid": "0000-0002-1443-8666"
    },
    {
      "affs": [
        0
      ],
      "name": "Ping-Chung Kuo",
      "orcid": "0000-0003-3019-1138"
    },
    {
      "affs": [
        1
      ],
      "name": "Dian Handayani"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4yjfqgny25py7.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c07232",
  "fulltext_url": "https://europepmc.org/articles/PMC13688500",
  "id": "4yjfqgny25py7",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/11/41/63520/5445676/Antibacterial-Activity-of-Equisetin-from",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2736|Microbial Natural Products and Biosynthesis"
    ]
  ],
  "openalex": "W7220692878",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c07232",
  "pmcid": "PMC13688500",
  "pmid": "42923996",
  "published": "2026-10-06",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/4yjfqgny25py7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c07232",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/41/63520/5445676/Antibacterial-Activity-of-Equisetin-from"
    },
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42923996",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42923996"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Microbial Natural Products and Biosynthesis",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Antibacterial Activity of Equisetin from Endophytic Fusarium incarnatum JMB4 Associated with Jatropha multifida L. Against MRSA: Experimental and In Silico Insights",
  "title_text": "Antibacterial Activity of Equisetin from Endophytic Fusarium incarnatum JMB4 Associated with Jatropha multifida L. Against MRSA: Experimental and In Silico Insights",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/4yjfqgny25py7",
  "venue": "ACS Omega",
  "versions": []
}