{
  "abstract": "Interferon-induced antiviral proteins act on the first line of defence against viruses, including influenza A virus (IAV). Among those, the short isoform of Nuclear Receptor Coactivator 7 (NCOA7-AS) has been shown to inhibit IAV entry and more especially the fusion between endosomal and viral membranes. Ectopic expression of NCOA7-AS leads to the increased acidification of the endolysosomal pathway, putatively through NCOA7-AS interaction with the vacuolar ATPase (V-ATPase). However, the precise mechanism of action of NCOA7-AS is not fully understood. Here, we identified the cellular partners of NCOA7-AS by mass spectrometry, including the V-ATPase subunits known to interact with NCOA7-AS. A point mutation disrupting the interaction with the V-ATPase led to loss of antiviral activity against IAV, demonstrating that V-ATPase engagement is required for the antiviral phenotype. Moreover, sorting nexin (SNX) 1/2/5/6 were identified as novel partners of NCOA7-AS. These proteins are involved in retrograde transport of cellular cargoes from endosomes to the trans-Golgi network. We revealed that SNX5/6 interacted directly with NCOA7-AS and were essential for NCOA7-AS antiviral activity against IAV. Interestingly, crystal structures of NCOA7-AS/SNX5 complexes showed that the SNX5-interaction motif in NCOA7-AS was similar to those found in known cargoes of SNX5/6. In addition, we could pinpoint several critical residues that were important for binding and antiviral activity. Collectively, our study identifies novel essential partners for NCOA7-AS antiviral activity and the structural basis for their interaction.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Mary Arnaud-Arnould"
    },
    {
      "name": "Antoine Rebendenne"
    },
    {
      "name": "Marine Tauziet"
    },
    {
      "name": "Serge Urbach"
    },
    {
      "name": "Khadija El Koulali"
    },
    {
      "name": "Emiliano P Ricci"
    },
    {
      "name": "Mélanie Wencker"
    },
    {
      "name": "Olivier Moncorgé"
    },
    {
      "name": "Mickaël Blaise"
    },
    {
      "name": "Caroline Goujon"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4za3mzhhf3fjv.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jbc.2026.113372",
  "fulltext_url": null,
  "id": "4za3mzhhf3fjv",
  "issns": [
    "0021-9258",
    "1083-351X"
  ],
  "landing_url": "https://hal.science/hal-05722248v2",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bc"
    ],
    [
      "hal",
      "1.sdv.mp"
    ],
    [
      "hal",
      "2.sdv.mp.vir"
    ],
    [
      "openalex",
      "2713|Influenza Virus Research Studies"
    ]
  ],
  "openalex": "W4409067184",
  "pdf_url": "https://hal.science/hal-05722248/document",
  "pmcid": null,
  "pmid": "42520939",
  "published": "2026-09-24",
  "publisher": "American Society for Biochemistry and Molecular Biology",
  "ris": "https://research.draconitic.com/paper/4za3mzhhf3fjv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:40Z",
      "native_id": "hal-05722248",
      "source": "hal",
      "url": "https://hal.science/hal-05722248v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.bc",
      "subfield": "cell-biology"
    },
    {
      "field": "biology",
      "origin": "hal:1.sdv.mp",
      "subfield": "microbiology"
    }
  ],
  "title": "SNX-BAR proteins 5 and 6 are required for NCOA7-AS antiviral activity against influenza A virus",
  "title_text": "SNX-BAR proteins 5 and 6 are required for NCOA7-AS antiviral activity against influenza A virus",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:40Z",
  "url": "https://research.draconitic.com/paper/4za3mzhhf3fjv",
  "venue": "Journal of Biological Chemistry",
  "versions": []
}