{
  "abstract": "Endocrine crosstalk between the liver and the brain regulates systemic energy homeostasis. However, this communication has not been comprehensively evaluated because identification of liver-derived proteins, or hepatokines, is difficult to define using bulk serum proteomics alone. To overcome this problem, we applied hepatocyte-restricted endoplasmic-reticulum targeted TurboID (ER-TurboID) in vivo and recovered biotinylated, liver-secretion competent proteins directly from specific brain regions, including the hypothalamus and hindbrain (nucleus of the solitary tract (NTS) and area postrema (AP)). Compared with AAV8-Null transduced controls, TurboID-expressing animals showed 3- to 21-fold enrichment of liver-expressed and predicted liver-secreted proteins among brain regions, with 81 canonical liver-secretome proteins, including multiple carboxylesterase (CES) family members. These results establish a method to identify novel secreted factors from peripheral tissues that can potentially act on specific brain regions.",
  "abstract_shown": true,
  "affiliations": [
    "University of Oklahoma Health Sciences"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "M. C. Juber"
    },
    {
      "name": "A. Asghari"
    },
    {
      "name": "K. E. Claflin"
    },
    {
      "affs": [
        0
      ],
      "name": "M. J. Potthoff"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4zjphkgdwd7rg.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.03.749208",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749208v1.full",
  "id": "4zjphkgdwd7rg",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749208v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1307|Biotin and Related Studies"
    ]
  ],
  "openalex": "W7211918771",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749208v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-03",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/4zjphkgdwd7rg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:55Z",
      "native_id": "10.64898/2026.09.03.749208",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.03.749208v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Biotin and Related Studies",
      "subfield": "cell-biology"
    }
  ],
  "title": "Method to Identify Liver-to-Brain Region-Specific Crosstalk Using TurboID",
  "title_text": "Method to Identify Liver-to-Brain Region-Specific Crosstalk Using TurboID",
  "type": "preprint",
  "updated_at": "2026-10-30T12:19:55Z",
  "url": "https://research.draconitic.com/paper/4zjphkgdwd7rg",
  "venue": "bioRxiv",
  "versions": []
}