{
  "abstract": "This research aimed to determine the performance of an in house dual western blot (WB) for the individual and simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies; the strip was designed with HTLV-1 antigen (1.67 ug/mm) on top and HIV-1 (1.34 ug/mm) on the bottom. The in-house dual WB was evaluated using 260 reference serum samples from a serum bank: 50 HTLV-1/HIV-1 positive, 50 HIV-1 positive, 60 HTLV-1 positive, 50 negative, and 50 potentially interfering samples. Accuracy was evaluated in five regions of Peru. Overall sensitivity and specificity were 96.3% (95% CI: 93.0‒99.5) and 100% (95% CI: 99.5‒100.0), respectively. Individually, sensitivity was 94% in HTLV-1/HIV-1 coinfected individuals, 96.7% in HTLV-1, and 98% in HIV-1. Repeatability and reproducibility were 100% under conditions of 34‒3256 m a.s.l., 16.4‒37.9 °C, and 42‒95% relative humidity. We conclude that the in house dual HTLV-1/HIV-1 WB shows high diagnostic performance.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratorio de Referencia Nacional de Virus de Transmisión Sexual, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Peru. Laboratorio de Referencia Nacional de Virus de Transmisión Sexual Centro Nacional de Salud Pública Instituto Nacional de Salud Lima Peru"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Eduardo Miranda-Ulloa",
      "orcid": "0000-0001-5687-5437"
    },
    {
      "affs": [
        0
      ],
      "name": "Soledad Romero-Ruiz",
      "orcid": "0000-0002-1360-9582"
    },
    {
      "affs": [
        0
      ],
      "name": "Ronal Briceño-Espinoza",
      "orcid": "0000-0002-0161-5782"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrés Vásquez-Domínguez",
      "orcid": "0000-0002-7554-3793"
    },
    {
      "affs": [
        0
      ],
      "name": "Dilan Suárez-Agüero",
      "orcid": "0000-0003-4440-1765"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/4zjw5a5qcssje.bib",
  "cited_by": 0,
  "doi": "10.17843/rpmesp.2026.433.16741",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13718571/",
  "id": "4zjw5a5qcssje",
  "issns": [
    "1726-4634",
    "1726-4642"
  ],
  "landing_url": "https://doi.org/10.17843/rpmesp.2026.433.16741",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Brief Report"
    ],
    [
      "openalex",
      "2406|HIV Research and Treatment"
    ]
  ],
  "openalex": "W7220441873",
  "pdf_url": null,
  "pmcid": "PMC13718571",
  "pmid": "42867957",
  "published": "2026-09-28",
  "publisher": "Instituto Nacional de Salud",
  "ris": "https://research.draconitic.com/paper/4zjw5a5qcssje.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T12:22:34Z",
      "native_id": "PMC13718571",
      "source": "pmc",
      "url": "https://doi.org/10.17843/rpmesp.2026.433.16741"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2406|HIV Research and Treatment",
      "subfield": "microbiology"
    }
  ],
  "title": "Diagnostic performance of a dual western blot for the simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies",
  "title_text": "Diagnostic performance of a dual western blot for the simultaneous detection of anti-HTLV-1 and anti-HIV-1 antibodies",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:34Z",
  "url": "https://research.draconitic.com/paper/4zjw5a5qcssje",
  "venue": "Revista Peruana de Medicina Experimental y Salud Publica",
  "versions": []
}