{
  "abstract": "Background/Objectives: Rapid identification of Staphylococcus aureus and differentiation of methicillin-resistant S. aureus (MRSA) remains critical to infection control and antimicrobial stewardship, particularly where rapid screening is desirable. In this study, we developed and evaluated a duplex recombinase polymerase amplification coupled with lateral flow dipstick (RPA–LFD) platform enabling simultaneous detection of the species-associated spa locus and the methicillin resistance determinant mecA within a single isothermal reaction. Methods: Primer–probe architecture and reaction parameters were systematically adjusted, with 39 °C selected as the operating temperature, to enable concurrent visual detection of both targets within 15 min. Results: In a preliminary ten-fold serial dilution experiment, visible detection was observed down to 10¹ CFU/mL for both targets, whereas conventional PCR produced visible amplification down to 10² CFU/mL under the respective assay conditions. This difference represents an assay-specific observation and was not used to establish a statistically validated detection limit. No cross-reactivity was observed against a panel of non-target bacterial species. A preliminary evaluation of 25 nasal swab-derived DNA specimens showed complete concordance with reference PCR within this limited sample set. Conclusions: These findings demonstrate the feasibility of concurrent visual detection of species-associated and resistance targets within a single RPA–LFD configuration. Although further analytical and clinical validation is required, the platform provides a technical framework for simplified duplex isothermal detection of S. aureus and mecA.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Clinical Pathology, Faculty of Medicine, , Navamindradhiraj University, Bangkok 10300, Thailand;",
    "Department of Pathology, Faculty of Medicine, , Nakhon Nayok 26120, Thailand; (S.S.); (K.O.)",
    "Center of Excellence in Medical and Environmental Innovation Research (CEMEIR), , Bangkok 10110, Thailand"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jutturong Ckumdee"
    },
    {
      "affs": [
        1
      ],
      "name": "Somchai Santiwatanakul"
    },
    {
      "affs": [
        1
      ],
      "name": "Kwanchai Onruang"
    },
    {
      "affs": [
        2
      ],
      "name": "Thongchai Kaewphinit"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/538e5x8359d47.bib",
  "cited_by": 0,
  "doi": "10.3390/diagnostics16203295",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13703673/?tool=EBI",
  "id": "538e5x8359d47",
  "issns": [
    "2075-4418"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13703673",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "R5-920"
    ],
    [
      "openalex",
      "2204|Biosensors and Analytical Detection"
    ]
  ],
  "openalex": "W7221511762",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13703673/pdf/?tool=EBI",
  "pmcid": "PMC13703673",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/538e5x8359d47.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:34Z",
      "native_id": "PMC:PMC13703673",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13703673"
    },
    {
      "first_seen": "2026-10-31T14:21:26Z",
      "native_id": "02c854c3c3bb4473884af431bd867ff8",
      "source": "doaj",
      "url": "https://doi.org/10.3390/diagnostics16203295"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "lcc:R5-920",
      "subfield": "general"
    }
  ],
  "title": "A Duplex Isothermal RPA–Lateral Flow Platform for Concurrent Detection of Staphylococcus Aureus and mecA-Mediated Methicillin Resistance",
  "title_text": "A Duplex Isothermal RPA–Lateral Flow Platform for Concurrent Detection of Staphylococcus Aureus and mecA-Mediated Methicillin Resistance",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T14:21:26Z",
  "url": "https://research.draconitic.com/paper/538e5x8359d47",
  "venue": "Diagnostics (Basel, Switzerland)",
  "versions": []
}