{
  "abstract": "The age-related decline in oocyte quality is a major risk factor for female infertility. Although transcriptome has been examined in aged oocytes, proteomic landscape, which reflects primary gene functional executors, remains largely unexplored. This gap limits our understanding of oocyte aging. To address this, we perform single-cell proteome/transcriptome co-profiling of GV/MII-aged oocytes from mice and humans, revealing species- and stage-specific proteomic/transcriptomic changes during oocyte aging. Strikingly, we observe uncoupled proteomic and transcriptomic alterations, indicating that proteomic changes in aged oocytes are not primarily driven by RNA levels. Furthermore, we capture molecular heterogeneity in aged oocytes and identify MCT4 as a candidate marker of oocyte aging. Functional studies suggest MCT4 contributes to aging-associated oocyte defects via lactate export, and its inhibition improves aged-oocyte quality. These findings indicate altered lactate metabolism as a candidate marker and intervention target for oocyte aging and underscore the value of our profiling in dissecting oocyte aging.",
  "abstract_shown": true,
  "affiliations": [
    "First Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China.",
    "Institute of Microanalytical Systems, Department of Chemistry, Key Laboratory of Excited-State Materials of Zhejiang Province, Zhejiang University, Hangzhou, China.",
    "School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.",
    "Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China.",
    "Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.",
    "Liangzhu Laboratory of Zhejiang University, Hangzhou, China.",
    "Department of Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, China.",
    "First Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China. yongcheng@zju.edu.cn.",
    "Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China. 3406028@zju.edu.cn.",
    "Institute of Microanalytical Systems, Department of Chemistry, Key Laboratory of Excited-State Materials of Zhejiang Province, Zhejiang University, Hangzhou, China. fangqun@zju.edu.cn.",
    "Single-cell Proteomics Research Center, ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, China. fangqun@zju.edu.cn.",
    "Key Laboratory for Biomedical Engineering of Ministry of Education, Cancer Center, Zhejiang University, Hangzhou, China. fangqun@zju.edu.cn.",
    "First Affiliated Hospital, Zhejiang University School of Medicine, and Liangzhu Laboratory of Zhejiang University, Hangzhou, China. xudongfu@zju.edu.cn.",
    "Institute of Hematology, Zhejiang University, Hangzhou, China. xudongfu@zju.edu.cn.",
    "Department of Geriatrics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. xudongfu@zju.edu.cn."
  ],
  "article_type": "research support, non u.s. gov't",
  "arxiv": null,
  "author_total": 21,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lanrui Cao"
    },
    {
      "affs": [
        0
      ],
      "name": "Hao Wu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yirong Jiang",
      "orcid": "0000-0001-7070-4909"
    },
    {
      "affs": [
        0
      ],
      "name": "Yaxue Hou"
    },
    {
      "affs": [
        2
      ],
      "name": "Zhuo Yang"
    },
    {
      "affs": [
        3
      ],
      "name": "Yuan Wang"
    },
    {
      "affs": [
        4
      ],
      "name": "Peipei Ren"
    },
    {
      "affs": [
        4
      ],
      "name": "Panpan Zhao"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaofeng Ni"
    },
    {
      "affs": [
        5
      ],
      "name": "Shuai Liu",
      "orcid": "0000-0002-8405-6743"
    },
    {
      "affs": [
        5
      ],
      "name": "Na Kong"
    },
    {
      "affs": [
        5
      ],
      "name": "Xin Sheng",
      "orcid": "0000-0002-3620-3577"
    },
    {
      "affs": [
        4
      ],
      "name": "Yinli Zhang",
      "orcid": "0000-0001-8353-9615"
    },
    {
      "affs": [
        4
      ],
      "name": "Songying Zhang",
      "orcid": "0000-0001-8615-7196"
    },
    {
      "affs": [
        6
      ],
      "name": "Jianfeng Xiao"
    },
    {
      "affs": [
        3
      ],
      "name": "Cunqi Ye",
      "orcid": "0000-0003-3117-8680"
    },
    {
      "affs": [
        4
      ],
      "name": "Heng-Yu Fan",
      "orcid": "0000-0003-4544-4724"
    },
    {
      "affs": [
        7
      ],
      "name": "Yongcheng Wang",
      "orcid": "0000-0003-2820-4243"
    },
    {
      "affs": [
        8
      ],
      "name": "Xiaomei Tong",
      "orcid": "0000-0002-6982-445X"
    },
    {
      "affs": [
        9,
        10,
        11
      ],
      "name": "Qun Fang",
      "orcid": "0000-0002-6250-252X"
    },
    {
      "affs": [
        12,
        13,
        14
      ],
      "name": "Xudong Fu",
      "orcid": "0000-0001-7024-0200"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/53brdyazfdcvd.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-77910-5",
  "fulltext_url": "https://europepmc.org/articles/PMC13683357",
  "id": "53brdyazfdcvd",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://europepmc.org/article/MED/42901925",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2739|Reproductive Biology and Fertility"
    ]
  ],
  "openalex": "W7213929895",
  "pdf_url": "https://europepmc.org/articles/PMC13683357?pdf=render",
  "pmcid": "PMC13683357",
  "pmid": "42901925",
  "published": "2026-09-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/53brdyazfdcvd.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:41Z",
      "native_id": "MED:42901925",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42901925"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2739|Reproductive Biology and Fertility",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Single-oocyte proteome-transcriptome co-profiling reveals a role of dysregulated lactate metabolism in oocyte aging.",
  "title_text": "Single-oocyte proteome-transcriptome co-profiling reveals a role of dysregulated lactate metabolism in oocyte aging.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:41Z",
  "url": "https://research.draconitic.com/paper/53brdyazfdcvd",
  "venue": "Nature communications",
  "versions": []
}