{
  "abstract": "Carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM-1) is a platelet receptor recognized as a negative regulator of platelet activity. During thrombus formation, platelets differentiate into distinct populations, but whether CEACAM-1 is preferentially associated with specific platelet phenotypes remains unclear. Here, we investigated CEACAM-1 localization in different platelet populations, focusing on the accessibility of its extracellular domain. In platelets adherent to poly-L-lysine, the CEACAM-1 signal was predominantly intracellular and decreased following treatment with acetylsalicylic acid (ASA), whereas glucose increased this signal. During integrin-dependent spreading on fibrinogen, CEACAM-1 was undetectable by immunofluorescence, irrespective of αIIbβ3 inhibition with tirofiban, although its presence was confirmed by Western blotting. Flow cytometry revealed that ASA reduced the percentage of CEACAM-1-positive platelets following ADP stimulation under both normoglycemic and hyperglycemic conditions. Confocal microscopy demonstrated co-localization of CEACAM-1 with phosphatidylserine (PS)-positive regions, a hallmark of the procoagulant platelet phenotype. In thrombi formed on collagen fibers under flow, ASA reduced the CEACAM-1-positive area under both conditions. However, a reduction in the ratio of CEACAM-1-positive area to PS-exposing area was observed only under normoglycemic conditions. In fibrin–platelet clots, CEACAM-1 was detected only after prolonged clot incubation and subsequent platelet permeabilization. Collectively, these findings reveal differences in CEACAM-1 localization and detectability across platelet populations and experimental settings, suggesting an association with platelet functional specialization.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biopharmacy and Radiopharmacy, Medical University of Bialystok, 15-222 Bialystok, Poland",
    "Radiopharmacy Centre, Medical University of Bialystok, 15-569 Bialystok, Poland",
    "Department of Physical Chemistry, Medical University of Bialystok, 15-089 Bialystok, Poland"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Natalia Bielicka",
      "orcid": "0000-0003-1454-647X"
    },
    {
      "affs": [
        1
      ],
      "name": "Adam Jan Olichwier",
      "orcid": "0000-0002-7989-0207"
    },
    {
      "affs": [
        2
      ],
      "name": "Tomasz Misztal",
      "orcid": "0000-0002-5403-9467"
    },
    {
      "affs": [
        0
      ],
      "name": "Magdalena Bruzgo-Grzybko",
      "orcid": "0009-0004-7614-8957"
    },
    {
      "affs": [
        1
      ],
      "name": "Izabela Suwda Kalita"
    },
    {
      "affs": [
        0
      ],
      "name": "Ewa Chabielska"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Anna Gromotowicz-Popławska"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/53pnshrkkwr4s.bib",
  "cited_by": 0,
  "doi": "10.3390/cells15211920",
  "fulltext_url": null,
  "id": "53pnshrkkwr4s",
  "issns": [
    "2073-4409"
  ],
  "landing_url": "https://www.mdpi.com/2073-4409/15/21/1920",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2720|Platelet Disorders and Treatments"
    ]
  ],
  "openalex": "W7223177514",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-22",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/53pnshrkkwr4s.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:02Z",
      "native_id": "10.3390/cells15211920",
      "source": "crossref",
      "url": "https://www.mdpi.com/2073-4409/15/21/1920"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "issn:2073-4409",
      "subfield": "cell-biology"
    }
  ],
  "title": "Differential Localization of CEACAM-1 in Distinct Platelet Populations",
  "title_text": "Differential Localization of CEACAM-1 in Distinct Platelet Populations",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:02Z",
  "url": "https://research.draconitic.com/paper/53pnshrkkwr4s",
  "venue": "Cells",
  "versions": []
}