{
  "abstract": "Background. Primary cilia coordinate Hedgehog-dependent outflow tract morphogenesis. Concurrent human genetics implicates ciliary loss-of-function in nonsyndromic tetralogy of Fallot (TOF) risk, yet compartment-resolved ciliary transcriptional programmes in public TOF snRNA-seq remain unmapped at module resolution. Methods. We curated a Cardiac Ciliary Transcriptomic Panel (CCTP; 1,611 genes; four modules) and executed a ten-phase integrative pipeline on six public sc/snRNA-seq resources. Differentially expressed gene tables were harmonised (adjusted p < 0.05; |log2FC| [≥] 0.5 where available), mapped to CCTP modules, and tiered by evidence strength. Patient-level pseudobulk (three TOF versus five donors), permutation-validated Compartment Dysregulation Index (CMDI), Cross-Species Concordance Score (CSCS), STRING protein-protein interaction analysis, and diffusion pseudotime on 17,290 Ho organoid cells were performed. Results. Among 1,263 unique CCTP-associated hits, 396 genes recurred in at least two dataset tables (exploratory tier; Tier A endothelial anchor n = 24 from Zulibiya et al.). Only Zulibiya endothelial M1 structural-assembly CMDI exceeded a module-size-matched permutation null (p = 0.015). Patient-level pseudobulk returned zero false-discovery-rate-significant CCTP genes. CCTP-conditioned overlap with recent ciliary genetics was non-significant (Fisher p {approx} 0.13). Ho organoid pseudotime showed highest M1/M2 activity at day 8, declining toward day 21. Genome-wide CSCS classified 11 genes as fully conserved and 136 as partially conserved among scoreable orthologs. Conclusions. Ciliary transcriptional correlates in TOF are compartment-specific and module-structured, with the strongest inferential signal in TOF endothelium (M1). CCTP-Atlas provides a reproducible, tiered prioritisation resource linking public TOF snRNA-seq, organoid maturation proxies, and partial cross-species patterns. Findings are hypothesis-generating and complement but do not replicate recent ciliary genetics evidence until expanded cohorts and functional validation are performed. Data availability. Processed gene lists, manifests, and scripts. Source datasets: GSE203274, GSE168464, GSE166462, GSE126128; Li et al. (2025) supplementary tables. This is a preprint and has not been peer-reviewed.",
  "abstract_shown": true,
  "affiliations": [
    "Riphah International University, Faisalabad"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "R. Tahir"
    },
    {
      "name": "T. Javed"
    },
    {
      "name": "M. B. Rasheed"
    },
    {
      "name": "Q. Aslam"
    },
    {
      "affs": [
        0
      ],
      "name": "N. Afsheen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/53rgxaww7gpbr.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.12.758603",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.12.758603v1.full",
  "id": "53rgxaww7gpbr",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.12.758603v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioinformatics"
    ],
    [
      "openalex",
      "1312|Congenital heart defects research"
    ]
  ],
  "openalex": "W7222937544",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.12.758603v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-12",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/53rgxaww7gpbr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:36Z",
      "native_id": "10.64898/2026.10.12.758603",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.12.758603v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioinformatics",
      "subfield": "bioinformatics"
    }
  ],
  "title": "CCTP-Atlas: Compartment-Resolved Ciliary Transcriptional Dysregulation in Tetralogy of Fallot, A Six-Dataset Integrative Meta-Analysis",
  "title_text": "CCTP-Atlas: Compartment-Resolved Ciliary Transcriptional Dysregulation in Tetralogy of Fallot, A Six-Dataset Integrative Meta-Analysis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:07Z",
  "url": "https://research.draconitic.com/paper/53rgxaww7gpbr",
  "venue": "bioRxiv",
  "versions": []
}