{
  "abstract": "Plants have remarkable capacity to reconstruct entire organ systems from tissue explants. In Arabidopsis two-step tissue culture system, pluripotency regulators are specifically expressed in the middle-cell layer of the stratified callus tissue. However, regulatory mechanisms underlying the radial patterning of callus remained unclear. Here, we found that very-long-chain fatty acids (VLCFAs) synthesized in the epidermis-like outermost layer are essential for pluripotency acquisition and successful shoot regeneration. Our genetic and transcriptomic analyses revealed that the regulatory roles of VLCFAs on pluripotency acquisition involve inter-cell layer signaling in callus tissue, while they are at least partly independent of ATML1/PDF2 functions and cuticular wax synthesis in the outermost layer. VLCFAs spatially restrict procambium cell identity by non-cell-autonomously suppressing cytokinin signaling, thereby allowing for establishment of the middle-cell layer. We propose that the inhibitory relationships between layer-specific regulators underlie the intricate balance of cellular fate determination in pluripotent callus.",
  "abstract_shown": true,
  "affiliations": [
    "Nara Institute of Science and Technology"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Y. Doll"
    },
    {
      "name": "T. Nobusawa"
    },
    {
      "name": "M. Kojima"
    },
    {
      "name": "K. Nagata"
    },
    {
      "name": "K. Matsuda-Ito"
    },
    {
      "name": "A. P. Mähönen"
    },
    {
      "name": "T. Matsuda"
    },
    {
      "name": "M. Abe"
    },
    {
      "name": "H. Sakakibara"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Ikeuchi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/54891amcnn39b.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.01.748728",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1.full",
  "id": "54891amcnn39b",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ],
    [
      "openalex",
      "1312|Plant tissue culture and regeneration"
    ]
  ],
  "openalex": "W7207546611",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/54891amcnn39b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:52Z",
      "native_id": "10.64898/2026.09.01.748728",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.01.748728v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus",
  "title_text": "VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus",
  "type": "preprint",
  "updated_at": "2026-10-30T12:18:52Z",
  "url": "https://research.draconitic.com/paper/54891amcnn39b",
  "venue": "bioRxiv",
  "versions": []
}