{
  "abstract": "Purpose\n\nThis study investigates the ability of Nanoskin, a bacterial cellulose (BC) biofilm, to transfer human corneal epithelial cells (CECs) onto wounded rabbit corneas, evaluating integration and re-epithelialization of the ocular surface.Methods\n\nPrimary cultures of human mesenchymal stem cells (MSCs) were established and differentiated into CECs, which were then seeded onto Nanoskin BC or soft contact lenses. Wounded rabbit corneas were covered with human CECs on BC biofilms or contact lenses and maintained in organ culture for 5 days. The control group was left untreated. Integration and re-epithelialization were assessed using immunofluorescence (IF) staining with human anti-nuclei antibodies.Results\n\nWounded rabbit corneas successfully re-epithelialized with both scaffolds. IF staining confirmed the presence of human epithelial cells in the regenerating rabbit corneal epithelium for both Nanoskin BC and contact lens groups, confirming effective cell transfer. Morphometric analysis revealed that control wounded corneas had significantly fewer epithelial cells (P < 0.001) compared to those treated with cell transfer.Conclusion\n\nOur findings demonstrate the feasibility of using Nanoskin BC as a scaffold for corneal cell transplantation, highlighting its potential as a promising biomaterial for treating ocular surface diseases. Future investigations are required to evaluate the transfer efficiency of MSCs, given their superior proliferative capacity and secretion of growth factors.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Ophthalmology, Medical School, São Paulo State University (Unesp), Botucatu, São Paulo, Brazil.",
    "Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (Unesp), Botucatu, São Paulo, Brazil.",
    "Department of Pathology, University of Sao Paulo - USP, Ribeirao Preto, SP, Brazil.",
    "Department of Orthopedics and Traumatology, Medical School, São Paulo State University (Unesp), Botucatu, São Paulo, Brazil.",
    "Innovatecs Biotechnological Research and Development, São Carlos, SP, Brazil.",
    "Department of Ophthalmology, University of New South Wales, Sydney, Australia."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Magda Viveiros"
    },
    {
      "affs": [
        1
      ],
      "name": "Cláudia Rainho"
    },
    {
      "affs": [
        1
      ],
      "name": "Natália Avelino"
    },
    {
      "affs": [
        1
      ],
      "name": "Carolina Gorgulho"
    },
    {
      "affs": [
        1
      ],
      "name": "Ramon Kaneno"
    },
    {
      "affs": [
        2
      ],
      "name": "Alexandre Fabro"
    },
    {
      "affs": [
        3
      ],
      "name": "Márcio Viveiros"
    },
    {
      "affs": [
        4
      ],
      "name": "Pierre Basmaji"
    },
    {
      "affs": [
        5
      ],
      "name": "Nick Di Girolamo"
    },
    {
      "affs": [
        0
      ],
      "name": "Silvana Schellini"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/54ge2z0yj774e.bib",
  "cited_by": 0,
  "doi": "10.18502/jovr.v21.18451",
  "fulltext_url": "https://europepmc.org/articles/PMC13711135",
  "id": "54ge2z0yj774e",
  "issns": [
    "2008-2010",
    "2008-322X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42948073",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "2741|Corneal Surgery and Treatments"
    ]
  ],
  "openalex": "W7220702267",
  "pdf_url": "https://europepmc.org/articles/PMC13711135?pdf=render",
  "pmcid": "PMC13711135",
  "pmid": "42948073",
  "published": "2026-10-06",
  "publisher": "Ophthalmic Research Center",
  "ris": "https://research.draconitic.com/paper/54ge2z0yj774e.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:34Z",
      "native_id": "MED:42948073",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42948073"
    },
    {
      "first_seen": "2026-10-31T12:21:58Z",
      "native_id": "PMC13711135",
      "source": "pmc",
      "url": "https://doi.org/10.18502/jovr.v21.18451"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Corneal Surgery and Treatments",
      "subfield": "imaging"
    }
  ],
  "title": "NanoskinⓇ Bacterial Cellulose as a Scaffold for De-epithelialized Corneal Surface Healing.",
  "title_text": "NanoskinⓇ Bacterial Cellulose as a Scaffold for De-epithelialized Corneal Surface Healing.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:58Z",
  "url": "https://research.draconitic.com/paper/54ge2z0yj774e",
  "venue": "Journal of ophthalmic & vision research",
  "versions": []
}