{ "abstract": "The study of redox biology has been growing constantly since the last decades. Over these years, redox processes have been linked to an extraordinarily wide range of physiological and pathological events, becoming recognized as central mechanisms underlying many of them. In this context, it becomes essential to understand the advantages and limitations of the tools under use, to recognize the specific controls required for each measurement and to accurately distinguish between distinct redox mechanisms. So far, multiple and excellent reviews have dealt with either the tools, the protocols or the mechanisms involved in reactive oxygen species (ROS) production and quenching, a.k.a. redox events. However, a review outlining the workflows to appropriately detect them is still lacking. We define workflow as the combination of tools, methods and mechanistic knowledge that allow the definition of a specific redox event. In this review, we aim to provide an optimal workflow for the research on mitochondrial redox events. To this end, we first summarize the molecular tools available to measure and quench ROS. We then explain the mechanisms of ROS production and scavenging in several of the cellular compartments, with special focus on mitochondria, as well as their implication in physiology and disease. Finally, we use the knowledge in all sections to build a recommended experimental workflow, illustrated by several cases of study. This review will enable the reader to understand how specific mitochondrial redox events can be accurately measured, considering all technical, methodological and mechanistical variables and limitations required for their reliable detection and interpretation.", "abstract_shown": true, "affiliations": [ "Centro de Neurociencias Cajal (CNC), Consejo Superior de Investigaciones Científicas (CSIC), Avenida de León 1, Alcalá de Henares, 28805, Spain; Corresponding author.", "Institute of Functional Biology and Genomics, University of Salamanca, CSIC, Salamanca, 37007, Spain; Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable-Instituto de Salud Carlos III (CIBERFES-ISCIII), Spain; Institute of Biomedical Research of Salamanca, University Hospital of Salamanca, University of Salamanca, CSIC, Salamanca, 37007, Spain", "Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable-Instituto de Salud Carlos III (CIBERFES-ISCIII), Spain; MiniAging/Freshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, Institute of Health Research-INCLIVA, Valencia, 46010, Spain", "Dept. Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Av. Alcalde Rovira Roure 80, Lleida, 25198, Spain; Institut de Recerca Biomèdica de Lleida Fundació Dr. Pifarré (IRBLleida), Av. Alcalde Rovira Roure 80, Lleida, 25198, Spain", "Department de Biomedical Sciences, School of Medicine and Health Sciences, University of Barcelona, Barcelona, 08036, Spain; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, 08036, Spain; Centro de Investigación Biomédica en Red Enfermedades Raras-Instituto de Salud Carlos III (CIBERER-ISCIII), Spain", "Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, 08003, Barcelona, 08908, Spain", "Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable-Instituto de Salud Carlos III (CIBERFES-ISCIII), Spain; Cardiovascular Regeneration Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, 28029, Spain", "TGF-β and Cancer Group, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, 08908, Spain; Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas-Instituto de Salud Carlos III (CIBERHED-ISCIII), Spain", "Institut de Biologia Molecular de Barcelona (IBMB) CSIC, Barcelona, Spain; CIBERDEM, Instituto de Salud Carlos III, Madrid, Spain", "Unidad de Investigación, Hospital Universitario Santa Cristina, Instituto de Investigación Sanitaria Princesa (IIS-Princesa), Madrid, 280009, Spain", "Instituto de Investigaciones Biomédicas Sols-Morreale (CSIC-UAM), Arturo Duperier, 4, Madrid, 28029, Spain", "Centro de Neurociencias Cajal (CNC), Consejo Superior de Investigaciones Científicas (CSIC), Avenida de León 1, Alcalá de Henares, 28805, Spain", "Department of Cell Biology and Functional Biology, Faculty of Biology, University of Valencia, Valencia, Spain", "Department of Physiology, Faculty of Pharmacy & Food Sciences, University of Valencia, Avda. Vicente Andrés Estellés 22, Burjasot, Valencia, 46100, Spain", "Institute for Research in Biomedicine (IRB Barcelona), Department of Biochemistry and Molecular Medicine, Universitat de Barcelona, Barcelona, 08028, Spain; CIBERDEM, Instituto de Salud Carlos III, Madrid, Spain" ], "article_type": null, "arxiv": null, "author_total": 18, "authors": [ { "affs": [ 0 ], "name": "Pablo Hernansanz-Agustín" }, { "affs": [ 1 ], "name": "Juan Pedro Bolaños" }, { "affs": [ 2 ], "name": "Consuelo Borrás" }, { "affs": [ 3 ], "name": "Elisa Cabiscol" }, { "affs": [ 4 ], "name": "Victoria Campuzano" }, { "affs": [ 5 ], "name": "Laura de Cubas" }, { "affs": [ 6 ], "name": "José Antonio Enríquez" }, { "affs": [ 7 ], "name": "Isabel Fabregat" }, { "affs": [ 7 ], "name": "Gemma Gonfaus-Ortiz" }, { "affs": [ 5 ], "name": "Elena Hidalgo" }, { "affs": [ 8 ], "name": "Marc Liesa" }, { "affs": [ 2 ], "name": "Cristina Mas-Bargues" }, { "affs": [ 9 ], "name": "Antonio Martínez-Ruiz" }, { "affs": [ 10 ], "name": "María Monsalve" }, { "affs": [ 11 ], "name": "Rubén Quintana-Cabrera" }, { "affs": [ 12 ], "name": "Sergio Rius-Pérez" }, { "affs": [ 13 ], "name": "Juan Sastre" }, { "affs": [ 14 ], "name": "Antonio Zorzano" } ], "bibtex": "https://research.draconitic.com/paper/55dxtmjjwkw2v.bib", "cited_by": 0, "doi": "10.1016/j.redox.2026.104387", "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2213231726003861", "id": "55dxtmjjwkw2v", "issns": [ "2213-2317" ], "landing_url": "https://doi.org/10.1016/j.redox.2026.104387", "language": "en", "licence": "CC-BY", "licence_label": "CC BY", "licence_source": "journal", "licence_url": "https://creativecommons.org/licenses/by/4.0/", "metadata_licence": null, "native_subjects": [ [ "lcc", "QH301-705.5" ], [ "lcc", "R5-920" ], [ "openalex", "1312|Redox biology and oxidative stress" ] ], "openalex": "W7212082967", "pdf_url": "https://www.sciencedirect.com/science/article/pii/S2213231726003861/pdf", "pmcid": null, "pmid": null, "published": "2026-10-30", "publisher": "Elsevier", "ris": "https://research.draconitic.com/paper/55dxtmjjwkw2v.ris", "sources": [ { "first_seen": "2026-10-30T13:43:16Z", "native_id": "f2c83240df874a01b1f5eb4a7682866c", "source": "doaj", "url": "https://doi.org/10.1016/j.redox.2026.104387" } ], "subjects": [ { "field": "biochemistry", "origin": "openalex:1312|Redox biology and oxidative stress", "subfield": "molecular-biology" } ], "title": "Experimental workflows for the accurate identification of mitochondrial redox events", "title_text": "Experimental workflows for the accurate identification of mitochondrial redox events", "type": "peer-reviewed", "updated_at": "2026-10-30T13:43:16Z", "url": "https://research.draconitic.com/paper/55dxtmjjwkw2v", "venue": "Redox Biology", "versions": [] }