{
  "abstract": "Trypanosoma cruzi, the causative agent of Chagas disease, exhibits a complex life cycle with multiple hosts, stages and differentiation steps. We present a cell atlas for the T. cruzi life cycle, based on single cell transcriptomes for over 31,000 cells and population-based transcriptomics. The atlas reveals many life cycle associated genes and can be utilised to accurately annotate life cycle stages. It provides detailed insights into cell heterogeneity, including cell-specific repertoires of surface antigens in trypomastigotes, with key implications for immune responses. Enabled by single-cell resolution, we define the transcriptomic changes that occur across the epimastigote to metacyclic trypomastigote differentiation axis. Furthermore, we provide comprehensive UTR annotation, identifying previously unannotated transcripts as well as revealing alternative polyadenylation and an unanticipated complexity of reverse strand and antisense transcripts. This T. cruzi atlas provides a comprehensive resource and unlocks a range of new avenues for research on this important human pathogen.",
  "abstract_shown": true,
  "affiliations": [
    "Leiden University Medical Centre, Leiden, The Netherlands",
    "School of Infection and Immunity, University of Glasgow, Glasgow, UK",
    "Drug Discovery Unit, Faculty of Life Sciences, University of Dundee, Dundee, UK",
    "Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany. ThomasDan.Otto@glasgow.ac.uk",
    "School of Infection and Immunity, University of Glasgow, Glasgow, UK. ThomasDan.Otto@glasgow.ac.uk",
    "University of Hamburg, Hamburg, Germany. ThomasDan.Otto@glasgow.ac.uk",
    "Drug Discovery Unit, Faculty of Life Sciences, University of Dundee, Dundee, UK. M.DeRycker@dundee.ac.uk"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Ross F Laidlaw",
      "orcid": "0009-0006-9559-7218"
    },
    {
      "affs": [
        2
      ],
      "name": "Marta García-Sánchez",
      "orcid": "0000-0001-5034-3384"
    },
    {
      "affs": [
        2
      ],
      "name": "Juliana da Silva Pacheco",
      "orcid": "0000-0002-7504-2120"
    },
    {
      "affs": [
        2
      ],
      "name": "Luciana S. Paradela"
    },
    {
      "affs": [
        3,
        4,
        5
      ],
      "name": "Thomas D. Otto",
      "orcid": "0000-0002-1246-7404"
    },
    {
      "affs": [
        6
      ],
      "name": "Manu De Rycker",
      "orcid": "0000-0002-3171-3519"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/56k13669rs2c7.bib",
  "cited_by": 10,
  "doi": "10.1038/s41467-026-73098-w",
  "fulltext_url": null,
  "id": "56k13669rs2c7",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://doi.org/10.1038/s41467-026-73098-w",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Trypanosoma species research and implications"
    ]
  ],
  "openalex": "W7162301917",
  "pdf_url": null,
  "pmcid": "PMC13385397",
  "pmid": "42185256",
  "published": "2026-05-25",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/56k13669rs2c7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:56Z",
      "native_id": "W7162301917",
      "source": "openalex",
      "url": "https://doi.org/10.1038/s41467-026-73098-w"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Trypanosoma species research and implications",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Trypanosoma cruzi cell atlas as a single-cell resource for understanding parasite population heterogeneity and differentiation",
  "title_text": "Trypanosoma cruzi cell atlas as a single-cell resource for understanding parasite population heterogeneity and differentiation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T13:20:39Z",
  "url": "https://research.draconitic.com/paper/56k13669rs2c7",
  "venue": "Nature Communications",
  "versions": []
}