{
  "abstract": "Background: Triple-negative breast cancer (TNBC) constitutes an invasive breast tumor subtype, marked by scarce available therapies and unfavorable patient prognosis. Natural products have attracted increasing attention as potential anticancer agents due to their multitarget activities and relatively low toxicity. This study aimed to investigate the antitumor effects of Biochanin A (BCA) in TNBC and to explore its underlying molecular mechanisms. Methods: The effects of BCA on TNBC cell proliferation, migration, and invasion were evaluated in vitro. Transcriptomic profiling, network pharmacology analysis, and mechanistic experiments were conducted to investigate the potential involvement of cuproptosis. The antimetastatic effects of BCA were further assessed in vivo using a mouse model. Results: BCA significantly inhibited the proliferative, migratory, and invasive capacities of TNBC cells. Integrative bioinformatics analyses identified cuproptosis as a potential mechanism underlying its antitumor activity. Mechanistic investigations suggested that BCA induces cuproptosis, accompanied by dysregulation of copper homeostasis, increased oxidative stress, mitochondrial dysfunction, and altered expression of cuproptosis-related proteins. Bioinformatics analysis further suggested that LIPT1 may be involved in this process. In vivo experiments demonstrated that BCA markedly suppressed lung metastasis in TNBC mouse models. Conclusions: BCA exhibits potent antitumor and antimetastatic activities against TNBC, potentially through the induction of cuproptosis, with LIPT1 possibly involved in this process. These findings provide experimental evidence supporting further investigation of BCA as a promising therapeutic candidate for TNBC.",
  "abstract_shown": true,
  "affiliations": [
    "West China School of Pharmacy, Sichuan University, Chengdu, China",
    "Department of Oncology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China",
    "The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China",
    "State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, China",
    "Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan",
    "Department of Urology, The Second Xiangya Hospital of Central South University, Changsha, China",
    "State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-Sen University Cancer Center, Guangzhou, China",
    "Department of Pharmacology, Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, Sichuan University, Chengdu, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Limei Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yue Duan"
    },
    {
      "affs": [
        0
      ],
      "name": "Shuang Pu"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Pingting Chen"
    },
    {
      "affs": [
        3
      ],
      "name": "Xiaofang Xie"
    },
    {
      "affs": [
        4
      ],
      "name": "Yiming Liu"
    },
    {
      "affs": [
        5
      ],
      "name": "Zhaokai Zhou"
    },
    {
      "affs": [
        6
      ],
      "name": "Hailin Tang"
    },
    {
      "affs": [
        1
      ],
      "name": "Lei Yu"
    },
    {
      "affs": [
        2
      ],
      "name": "Danping Chen"
    },
    {
      "affs": [
        0,
        7
      ],
      "name": "Fu Peng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/56m23z0qbte09.bib",
  "cited_by": 0,
  "doi": "10.32604/or.2026.084760",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13732377/",
  "id": "56m23z0qbte09",
  "issns": [
    "0965-0407",
    "1555-3906"
  ],
  "landing_url": "https://doi.org/10.32604/or.2026.084760",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1306|Ferroptosis and cancer prognosis"
    ]
  ],
  "openalex": "W7214970831",
  "pdf_url": null,
  "pmcid": "PMC13732377",
  "pmid": "42985375",
  "published": "2026-10-28",
  "publisher": "Tech Science Press",
  "ris": "https://research.draconitic.com/paper/56m23z0qbte09.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:22:22Z",
      "native_id": "PMC13732377",
      "source": "pmc",
      "url": "https://doi.org/10.32604/or.2026.084760"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|Ferroptosis and cancer prognosis",
      "subfield": "oncology"
    }
  ],
  "title": "Biochanin A Induces Cuproptosis and Inhibits Lung Metastasis in triple-negative Breast Cancer",
  "title_text": "Biochanin A Induces Cuproptosis and Inhibits Lung Metastasis in triple-negative Breast Cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:22:22Z",
  "url": "https://research.draconitic.com/paper/56m23z0qbte09",
  "venue": "Oncology Research",
  "versions": []
}