{
  "abstract": "Muscle loss accompanied by excessive adiposity is a critical component of metabolic dysfunction. This study investigated the effects of Lactiplantibacillus plantarum-fermented broccoli (FHB) in high-fat/high-fructose diet (HFFD)-fed C57BL/6J mice. LC-MS profiling showed that fermentation generated metabolites, including 2-hydroxycaproic acid, 3-phenyllactic acid, and a tetrahydro-β-carboline derivative. FHB supplementation attenuated visceral adiposity, hepatic steatosis, and insulin resistance while preserving grip strength. In the gastrocnemius muscle, FHB was associated with higher phosphorylation of the IRS1/PI3K/Akt and mTOR/p70S6K/4E-BP1 pathways and with lower expression of the ubiquitin ligases Atrogin-1 and MuRF-1. 16S rRNA sequencing showed altered gut microbiota, with enrichment of the butyrate-producing species Anaerostipes hominis and Bifidobacterium pseudolongum in the FHB group. These findings indicate that FHB supplementation was associated with attenuated metabolic dysfunction and preserved muscle function, accompanied by fermentation-derived metabolites and gut microbiota remodeling.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Food Sciences and Technology, National Taiwan University, Taipei10617, Taiwan.",
    "Research and Development Department, Syngen Biotech Co., Ltd., Tainan73055, Taiwan.",
    "Department of Medical Research, China Medical University Hospital, China Medical University, Taichung40402, Taiwan.",
    "Department of Health and Nutrition Biotechnology, Asia University, Taichung413305, Taiwan."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ya-Ru Kuo"
    },
    {
      "affs": [
        0
      ],
      "name": "Tzu-Ling Lin"
    },
    {
      "affs": [
        0
      ],
      "name": "Pin-Yu Ho"
    },
    {
      "affs": [
        1
      ],
      "name": "Wei-Jen Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Hui-Fang Chu"
    },
    {
      "affs": [
        1
      ],
      "name": "Ming-Chung Tseng"
    },
    {
      "affs": [
        1
      ],
      "name": "Yu-Shan Wei"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "Min-Hsiung Pan",
      "orcid": "0000-0002-5188-7030"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/56pjve07xjh0w.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.jafc.6c05322",
  "fulltext_url": "https://europepmc.org/articles/PMC13651633",
  "id": "56pjve07xjh0w",
  "issns": [
    "0021-8561",
    "1520-5118"
  ],
  "landing_url": "https://europepmc.org/article/MED/42915814",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Gut microbiota and health"
    ]
  ],
  "openalex": "W7214632176",
  "pdf_url": "https://europepmc.org/articles/PMC13651633?pdf=render",
  "pmcid": "PMC13651633",
  "pmid": "42915814",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/56pjve07xjh0w.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42915814",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42915814"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Gut microbiota and health",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Lactiplantibacillus plantarum-Fermented Broccoli Attenuates Metabolic Dysfunction and Improves Skeletal Muscle Quality in High-Fat/High-Fructose Diet-Fed Mice.",
  "title_text": "Lactiplantibacillus plantarum-Fermented Broccoli Attenuates Metabolic Dysfunction and Improves Skeletal Muscle Quality in High-Fat/High-Fructose Diet-Fed Mice.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/56pjve07xjh0w",
  "venue": "Journal of agricultural and food chemistry",
  "versions": []
}