{
  "abstract": "Homologous chromosomes are spatially segregated, or antipaired, in neonatal human endothelial cells at metaphase and anaphase, which might help prevent abnormal recombination. However, it is unclear whether this antipairing persists in adult endothelial cells. To test whether the antipairing, or one homolog per nuclear hemisphere motif, is conserved in adult endothelial cells, we examined human aortic endothelial cells at metaphase. Using immunolabeling with DNA fluorescent in situ hybridization (ImmunoFISH) and high-resolution confocal microscopy to visualize the chromosomes and centrosomes, we found that small homologous chromosomes 13, 15, 17, 19, 21, 22, and the sex chromosomes (XY) exhibit a loss of spatial segregation in human adult aortic endothelial cells. In addition, fewer adult endothelial cells showed segregation for the larger chromosomes 1, 4 and XX, as compared to in neonatal endothelial cells. Notably, we observed a higher frequency of abnormal pairing in both small and large chromosomes in adult aortic endothelial cells as compared to in neonatal umbilical vein endothelial cells. These findings suggest that mechanisms governing chromosome antipairing might decline with aortic endothelial cell age.",
  "abstract_shown": true,
  "affiliations": [
    "Biology Department, Sonoma State University, Rohnert Park, CA 94928, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jemery Morales"
    },
    {
      "affs": [
        0
      ],
      "name": "Omar Akhtar",
      "orcid": "0009-0007-2873-3715"
    },
    {
      "affs": [
        0
      ],
      "name": "Gabriel Quintero Plancarte",
      "orcid": "0009-0001-8476-1573"
    },
    {
      "affs": [
        0
      ],
      "name": "Lisa Hua",
      "orcid": "0009-0008-2975-2384"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/57g6kt2ajcvm7.bib",
  "cited_by": 3,
  "doi": "10.1242/jcs.264668",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13723388/",
  "id": "57g6kt2ajcvm7",
  "issns": [
    "0021-9533",
    "1477-9137"
  ],
  "landing_url": "https://doi.org/10.1242/jcs.264668",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1307|Microtubule and mitosis dynamics"
    ]
  ],
  "openalex": "W4408510119",
  "pdf_url": null,
  "pmcid": "PMC13723388",
  "pmid": "42836297",
  "published": "2026-10-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/57g6kt2ajcvm7.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:22:02Z",
      "native_id": "PMC13723388",
      "source": "pmc",
      "url": "https://doi.org/10.1242/jcs.264668"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Microtubule and mitosis dynamics",
      "subfield": "cell-biology"
    }
  ],
  "title": "Higher frequency of homologous chromosome pairing in adult endothelial cells as compared to in neonatal endothelial cells",
  "title_text": "Higher frequency of homologous chromosome pairing in adult endothelial cells as compared to in neonatal endothelial cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:13Z",
  "url": "https://research.draconitic.com/paper/57g6kt2ajcvm7",
  "venue": "Journal of Cell Science",
  "versions": []
}