{
  "abstract": "Objectives:: This study aimed to develop and evaluate an in-house flow cytometry-based direct antiglobulin test (DAT) for detecting IgG-bound platelets in cats and to investigate the prevalence of DAT positivity and associated risk factors among thrombocytopenic cats.\n\nMethods:: Platelets were identified by CD41/61 expression, and platelet-bound IgG was assessed using flow cytometry. A diagnostic cutoff for the percentage of IgG-positive platelets was established using 100 healthy cats and preliminarily evaluated in 50 cats with severe thrombocytopenia (platelet count <20,000/µl) before being applied to the remaining 411 thrombocytopenic cats (platelet count <200,000/µl) to determine the prevalence of DAT positivity and its associations with platelet count, breed, age, and sex.\n\nResults:: Healthy cats showed 0–10% IgG-positive platelets, and a cutoff of >10% was established. Using this cutoff, 105/461 (22.8%) thrombocytopenic cats were classified as DAT positive. DAT-positive cats had significantly lower platelet counts than DAT-negative cats (median 4000/µl; mean 12,562/µl vs 34,000/µl; mean 47,956/µl; P <0.05), and the percentage of IgG-bound platelets was inversely correlated with platelet count (r = −0.38; P = 0.048). No breed association was identified. Cats aged 5 years were more likely to be DAT positive, whereas castrated male cats were less likely to be DAT positive.\n\nConclusions and relevance:: Flow cytometry-based DAT is a useful adjunctive diagnostic tool for assessing suspected immune-mediated platelet destruction in thrombocytopenic cats. Younger cats may be at increased risk, whereas castrated male cats may be at lower risk. However, the interpretation of a DAT-positive test result should be made alongside assessment of clinical findings and investigations for underlying causes of thrombocytopenia.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand",
    "Medical Microbiology Interdisciplinary Program, College of Interdisciplinary and Integrative Studies, Chulalongkorn University, Bangkok, Thailand",
    "Veterinary Diagnostic Laboratory (CU VDL), Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand",
    "CU VET Blood Bank, Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Teerawut Nedumpun",
      "orcid": "0000-0002-9924-1036"
    },
    {
      "affs": [
        1
      ],
      "name": "Ousakorn Kamenkit"
    },
    {
      "affs": [
        2
      ],
      "name": "Sinittha Thongwandi"
    },
    {
      "affs": [
        0
      ],
      "name": "Thitima Tripipat"
    },
    {
      "affs": [
        3
      ],
      "name": "Suwarath Wadeerat"
    },
    {
      "affs": [
        2
      ],
      "name": "Supanat Boonyapisitsopa"
    },
    {
      "affs": [
        2
      ],
      "name": "Napawan Bunpapong"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/588h4j7y74f6d.bib",
  "cited_by": 0,
  "doi": "10.1177/1098612x261478821",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13735088/",
  "id": "588h4j7y74f6d",
  "issns": [
    "1098-612X",
    "1532-2750"
  ],
  "landing_url": "https://doi.org/10.1177/1098612x261478821",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "2720|Platelet Disorders and Treatments"
    ]
  ],
  "openalex": "W7202078469",
  "pdf_url": "https://journals.sagepub.com/doi/pdf/10.1177/1098612X261478821?download=true",
  "pmcid": "PMC13735088",
  "pmid": "42572226",
  "published": "2026-08-09",
  "publisher": "SAGE Publications",
  "ris": "https://research.draconitic.com/paper/588h4j7y74f6d.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:20:51Z",
      "native_id": "PMC13735088",
      "source": "pmc",
      "url": "https://doi.org/10.1177/1098612x261478821"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2720|Platelet Disorders and Treatments",
      "subfield": "general"
    }
  ],
  "title": "Flow cytometry-based direct antiglobulin test for suspected feline immune-mediated thrombocytopenia: Assay development and demographic factors associated with direct antiglobulin test positivity",
  "title_text": "Flow cytometry-based direct antiglobulin test for suspected feline immune-mediated thrombocytopenia: Assay development and demographic factors associated with direct antiglobulin test positivity",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:20:51Z",
  "url": "https://research.draconitic.com/paper/588h4j7y74f6d",
  "venue": "Journal of Feline Medicine and Surgery",
  "versions": []
}