{
  "abstract": "Non-coding RNAs play critical roles in regulating gene expression and diverse biological processes. While the roles of microRNAs (miRNAs/miRs) are well known, the roles of long non-coding RNAs (lncRNAs; >200 nucleotides, with little or no protein-coding potential) remain less defined. In this study, we investigated the function of lncRNAs during the differentiation of human retinal pigment epithelial (ARPE-19) cells, identifying LINC00276 as being consistently and robustly upregulated during RPE differentiation. Silencing LINC00276-202 (ENST00000418420.1) using antisense GapmeRs significantly reduced the expression of RPE differentiation-associated genes, including RPE65 , MITF , TRPM1 , and TRPM3 , whereas LINC00276-202 overexpression restored their expression levels. In silico analysis identified a shared miR-204-5p/miR-211-5p-responsive region within LINC00276-202 . Both miRNAs produced modest increases in LINC00276-202 -associated reporter activity, whereas deletion of the shared seed-recognition region attenuated this response. Importantly, despite increased accumulation of the seed-deletion transcript, the mutant construct failed to induce RPE65 expression to the extent observed with WT LINC00276-202 , indicating that sequence integrity contributes to its biological activity. RNA pull-down followed by mass spectrometry identified PURB, SART3, ANXA6, and hnRNPL as potential protein binding partners. Of these, hnRNPL was validated as a LINC00276-202 -interacting protein by RNA immunoprecipitation and immunoblotting. LINC00276-202 modulated hnRNPL protein levels, and hnRNPL contributed to LINC00276-202 -mediated regulation of β-catenin protein abundance and TCF/LEF transcriptional activity. Together, these findings identify LINC00276-202 as a novel regulator of RPE differentiation involving a miR-204/miR-211-responsive region, hnRNPL interaction, and β-catenin-associated signaling, with potential relevance to retinal degenerative disease.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Samuel William",
      "orcid": "0000-0003-2975-188X"
    },
    {
      "name": "Olga A. Postnikova",
      "orcid": "0000-0002-2340-4978"
    },
    {
      "name": "Sheetal Uppal",
      "orcid": "0000-0002-3685-8602"
    },
    {
      "name": "Tapan K. Maity",
      "orcid": "0009-0003-0184-0239"
    },
    {
      "name": "Lisa M. Jenkins",
      "orcid": "0000-0003-1245-1338"
    },
    {
      "name": "Todd Duncan"
    },
    {
      "name": "Ashish Lal"
    },
    {
      "name": "T. Michael Redmond",
      "orcid": "0000-0002-1813-5291"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/58m44rj06t9qe.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-74123-0",
  "fulltext_url": null,
  "id": "58m44rj06t9qe",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-74123-0",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1306|Cancer-related molecular mechanisms research"
    ]
  ],
  "openalex": "W7218782204",
  "pdf_url": "https://www.nature.com/articles/s41598-026-74123-0_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-03",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/58m44rj06t9qe.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:19Z",
      "native_id": "10.1038/s41598-026-74123-0",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-74123-0"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|Cancer-related molecular mechanisms research",
      "subfield": "oncology"
    }
  ],
  "title": "The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling",
  "title_text": "The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:19Z",
  "url": "https://research.draconitic.com/paper/58m44rj06t9qe",
  "venue": "Scientific Reports",
  "versions": []
}