{
  "abstract": "The precise classification of basal, classical, atypical, and mesenchymal subtypes of Head and Neck Squamous Cell Carcinoma (HNSCC) is crucial for advancing precision oncology. Our research aims to develop an explainable subtype classification model for HNSCC.These molecular subtypes have been previously defined; reliable and meaningful computational methods for the classification of molecular subtypes and prioritization of molecular biomarkers for each subtype do not exist. We developed an explainable artificial intelligence (XAI) model that classifies molecular subtypes based on combined transcriptomic profiles from The Cancer Genome Atlas (TCGA-HNSC) and Gene Expression Omnibus (GSE65858). SHapley Additive exPlanations (SHAP) were used to quantify the importance of genes for model predictions. Subsequently, genes with high predictive importance were analyzed in protein-protein interactions using STRING, and hub genes were identified by Maximum Clique Centrality (MCC) and used for the identification of candidate biomarkers for each of the subtypes. We employed machine learning methods such as Random Forest (RF), Logistic Regression (LR), Decision Tree (DT), AdaBoost (AB), Support Vector Machine (SVM), K-Nearest Neighbours (KNN), Gaussian Naive Bayes (GNB), XGBoost (XGB), and Gradient Boosting (GB). The XGBoost model achieved an accuracy of 95% after hyperparameter tuning and cross-validation. Biologically relevant subtype-specific biomarkers associated with each molecular subtype were identified. To highlight the potential biological functions of these biomarkers, pathway analysis was performed. Further assessment of these genes, such as survival analysis and druggability, showed promise for the development of targeted therapies.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Sciences, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Chennai, India",
    "School of Computer Science and Engineering, Vellore Institute of Technology, Vellore, Tamil Nadu, India",
    "Department of Computer Science and Engineering, Amrita School of Computing, Amrita Vishwa Vidyapeetham, Chennai, India"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Anagha Rajan"
    },
    {
      "affs": [
        1
      ],
      "name": "Christopher Leela Biji"
    },
    {
      "affs": [
        2
      ],
      "name": "Mangalam Goutam Sinha"
    },
    {
      "name": "Iyappan Ramalakshmi Oviya"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/58s5g2hegee0e.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0360612",
  "fulltext_url": null,
  "id": "58s5g2hegee0e",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0360612",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Biomarkers"
    ],
    [
      "plos",
      "/Biology and life sciences/Computational biology/Genome analysis/Transcriptome analysis"
    ],
    [
      "plos",
      "/Biology and life sciences/Developmental biology/Cell differentiation"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Genomics/Genome analysis/Transcriptome analysis"
    ],
    [
      "plos",
      "/Computer and information sciences/Artificial intelligence/Machine learning"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Carcinoma/Squamous cell carcinoma/Head and neck squamous cell carcinoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Head and neck cancers"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Head and neck cancers/Head and neck tumors/Head and neck squamous cell carcinoma"
    ],
    [
      "plos",
      "/Physical sciences/Mathematics/Statistics/Statistical methods/Survival analysis"
    ],
    [
      "plos",
      "/Research and analysis methods/Mathematical and statistical techniques/Statistical methods/Survival analysis"
    ],
    [
      "plos",
      "/Research and analysis methods/Research design/Clinical research design/Survival analysis"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-04",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/58s5g2hegee0e.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T12:20:11Z",
      "native_id": "10.1371/journal.pone.0360612",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0360612"
    }
  ],
  "subjects": [
    {
      "field": "mathematics",
      "origin": "plos:statistical methods",
      "subfield": "statistics"
    },
    {
      "field": "medicine",
      "origin": "plos:cancers and neoplasms",
      "subfield": "oncology"
    }
  ],
  "title": "Explainable computational framework for subtype classification and biomarker identification in head and neck squamous cell carcinoma using machine learning",
  "title_text": "Explainable computational framework for subtype classification and biomarker identification in head and neck squamous cell carcinoma using machine learning",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:11Z",
  "url": "https://research.draconitic.com/paper/58s5g2hegee0e",
  "venue": "PLOS One",
  "versions": []
}