{
  "abstract": "Introduction: Sleep deprivation is increasingly recognized as a major health concern and is associated with impaired physiological and neurological functions. Panax ginseng C. A. Mey. has long been used to improve sleep quality; however, the limited availability and long cultivation cycle of natural ginseng restrict its sustainable utilization. Wild ginseng adventitious roots (ADV), produced via plant tissue culture, provide a renewable source of bioactive ginsenosides, although their potential role in sleep regulation remains poorly understood.\n\nMethods: The sleep-promoting effects and safety of ADV were evaluated in a sleep deprivation-induced Drosophila melanogaster model. Sleep parameters, including total sleep duration, sleep latency, deep sleep, and sleep fragmentation, were assessed, together with addiction-like behavioral assays. Transcriptomic analysis was performed to investigate the underlying mechanisms. LC-MS/MS and bioactivity assays were conducted to identify and validate major active ginsenosides.\n\nResults: ADV significantly improved sleep behavior in sleep-deprived flies, increasing total sleep duration and deep sleep while reducing sleep latency and sleep fragmentation, without inducing addiction-like behaviors. Transcriptomic analysis revealed that ADV modulated multiple sleep-related biological processes, particularly stress response pathway, as evidenced by altered expression of Hsp68, Hsp70Aa, Hsp70Ba, Hsp70Bb, and Hsp70Bbb . Combined with LC-MS/MS identification and biological activity comparison, ginsenosides Rc and F2 were preliminarily identified as the principal active metabolites.\n\nConclusion: In the Drosophila melanogaster sleep-deprivation model, ADV treatment is associated with improvements in sleep-related behavioural parameters alongside altered expression of conserved stress-response-related biological processes. These findings support ADV as a sustainable source of bioactive ginsenosides, though the current experimental design cannot establish a strictly sleep-specific or causal mechanistic relationship.",
  "abstract_shown": true,
  "affiliations": [
    "Shanghai Jiao Tong University , ,",
    "Tonghua Herbal Biotechnology Co., Ltd. , ,",
    "The Affiliated Hospital to Changchun University of Chinese Medicine , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Hong Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiangzhu Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Hang Chi"
    },
    {
      "affs": [
        1
      ],
      "name": "Yongjun Zheng"
    },
    {
      "affs": [
        2
      ],
      "name": "Yingna Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Lu Xing"
    },
    {
      "affs": [
        2
      ],
      "name": "Rui Ma"
    },
    {
      "affs": [
        2
      ],
      "name": "Liwei Sun"
    },
    {
      "affs": [
        2
      ],
      "name": "Xuenan Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/58smmab701kah.bib",
  "cited_by": 0,
  "doi": "10.3389/fphar.2026.1907250",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13672481/?tool=EBI",
  "id": "58smmab701kah",
  "issns": [
    "1663-9812"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13672481",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Ginseng Biological Effects and Applications"
    ]
  ],
  "openalex": "W7216218815",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13672481/pdf/?tool=EBI",
  "pmcid": "PMC13672481",
  "pmid": null,
  "published": "2026-10-02",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/58smmab701kah.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:25Z",
      "native_id": "PMC:PMC13672481",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13672481"
    },
    {
      "first_seen": "2026-10-30T12:20:06Z",
      "native_id": "10.3389/fphar.2026.1907250",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fphar.2026.1907250/full"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Ginseng Biological Effects and Applications",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Wild ginseng adventitious roots restore sleep homeostasis in sleep-deprived Drosophila melanogaster",
  "title_text": "Wild ginseng adventitious roots restore sleep homeostasis in sleep-deprived Drosophila melanogaster",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:06Z",
  "url": "https://research.draconitic.com/paper/58smmab701kah",
  "venue": "Frontiers in pharmacology",
  "versions": []
}