{
  "abstract": "Background and objectives\n\nAcute pancreatitis-associated lung injury (AP-LI) is a severe complication with limited therapeutic options. This study investigated the role of plasma exosomes in AP-LI and the underlying molecular mechanism.Methods\n\nA murine AP-LI model and primary alveolar type II epithelial (AT2) cells were used. Small RNA sequencing, bioinformatic and enrichment analyses, functional assays, and in vivo and in vitro rescue experiments were performed to identify key exosomal miRNAs and define their downstream signaling pathways.Results\n\nPlasma exosomes aggravated AP-LI. Small RNA sequencing revealed marked alterations in exosomal miRNA profiles, with miR-25-3p identified as a key candidate associated with oxidative stress and ferroptosis. NADPH oxidase 4 (NOX4) was predicted and validated as a direct target of miR-25-3p. miR-25-3p suppressed NOX4 expression and attenuated ferroptosis in AT2 cells. Nuclear receptor coactivator 4 (NCOA4)-mediated ferritinophagy acted downstream of NOX4, promoted iron dysregulation, and reinforced ferroptotic injury. Rescue experiments confirmed the protective role of the miR-25-3p/NOX4/NCOA4 axis.Conclusions\n\nPlasma exosomal miR-25-3p alleviates AP-LI by repressing NOX4 and limiting NCOA4-mediated ferritinophagy and ferroptosis, suggesting a potential therapeutic target for pulmonary complications of acute pancreatitis.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Oncology Laparoscopic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.",
    "Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.",
    "Department of Nuclear Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.",
    "Department of Breast Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.",
    "Department of Gastrointestinal Surgery, The First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu, Anhui Province, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zixin Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zijian Huang"
    },
    {
      "affs": [
        2
      ],
      "name": "Jiafu Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Liang Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Long Cheng"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Meng He"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xuchen Hu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Junpeng Wu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yusen Feng"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Liang Ji",
      "orcid": "0009-0003-0493-1160"
    },
    {
      "affs": [
        4
      ],
      "name": "Yan Jin",
      "orcid": "0000-0001-5957-7443"
    },
    {
      "affs": [
        0
      ],
      "name": "Gang Wang",
      "orcid": "0000-0002-1186-4336"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/592bee5rdy6he.bib",
  "cited_by": 0,
  "doi": "10.1515/jtim-2026-0071",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13726662/?tool=EBI",
  "id": "592bee5rdy6he",
  "issns": [
    "2450-131X",
    "2224-4018"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13726662",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7223401715",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13726662/pdf/?tool=EBI",
  "pmcid": "PMC13726662",
  "pmid": "42974507",
  "published": "2026-10-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/592bee5rdy6he.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T15:20:51Z",
      "native_id": "PMC:PMC13726662",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13726662"
    },
    {
      "first_seen": "2026-11-04T15:20:48Z",
      "native_id": "MED:42974507",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42974507"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Plasma exosomal miR-25-3p alleviates acute pancreatitis-associated lung injury by inhibiting ferroptosis of type II alveolar epithelial cells via NOX4/NCOA4-mediated ferritinophagy",
  "title_text": "Plasma exosomal miR-25-3p alleviates acute pancreatitis-associated lung injury by inhibiting ferroptosis of type II alveolar epithelial cells via NOX4/NCOA4-mediated ferritinophagy",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T03:20:59Z",
  "url": "https://research.draconitic.com/paper/592bee5rdy6he",
  "venue": "Journal of translational internal medicine",
  "versions": []
}