{
  "abstract": "Affinity membranes are developed by the immobilization of heparin onto anodized aluminum oxide (AAO) supports via amine grafting followed by amide coupling. Optimized procedures give heparinized AAO (HEP-AAO) membranes with a stable layer of 0.832-1.33 mg heparin per m² pore area in 224 nm diameter pores. Following the heparinization of the membrane, the bioavailability of heparin is demonstrated through the capture and release of fibroblast growth factor 2 (FGF2), a protein for which heparin acts as a ligand. The capping of unreacted amines using citraconic anhydride reduces nonspecific protein binding without loss of heparin activity. The pore accessibility of colloidal particles is confirmed through the electrostatically-driven binding and release of 20 nm fluorescent amine-grafted silica nanoparticles, which are used as a model system to investigate the capture and release of biocolloids. Application of heparinized AAO membranes for the affinity capture and release of adeno associated virus 2 (AAV-2) is demonstrated in tangential flow filtration. Preliminary results for the affinity capture of AAV-2 pre-filtered culture medium capture significant AAV2 with negligible retention of host protein and DNA, paving a path for further optimization of viral vector purification.",
  "abstract_shown": true,
  "affiliations": [
    "University of Kentucky, Department of Chemical & Materials Engineering Lexington KY 40406-0046 USA stephen.rankin@uky.edu bknut2@uky.edu.",
    "University of Kentucky, Department of Molecular and Cellular Biochemistry Lexington KY 40506 USA."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kayla E Daugherty",
      "orcid": "0009-0004-0389-6196"
    },
    {
      "affs": [
        0
      ],
      "name": "Logan D Martin",
      "orcid": "0009-0006-7495-4634"
    },
    {
      "affs": [
        0
      ],
      "name": "Mara Leach-Baughman"
    },
    {
      "affs": [
        1
      ],
      "name": "Kearstin L Edmonds"
    },
    {
      "affs": [
        1
      ],
      "name": "Rebecca E Dutch"
    },
    {
      "affs": [
        0
      ],
      "name": "Malgorzata Chwatko",
      "orcid": "0000-0002-2770-5196"
    },
    {
      "affs": [
        0
      ],
      "name": "Stephen E Rankin",
      "orcid": "0000-0002-8615-7564"
    },
    {
      "affs": [
        0
      ],
      "name": "Barbara L Knutson",
      "orcid": "0000-0001-5606-6226"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/59b43tp9b12g4.bib",
  "cited_by": 0,
  "doi": "10.1039/d6ra07687d",
  "fulltext_url": "https://europepmc.org/articles/PMC13682590",
  "id": "59b43tp9b12g4",
  "issns": [
    "2046-2069"
  ],
  "landing_url": "https://europepmc.org/article/MED/42903969",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Chemistry"
    ],
    [
      "openalex",
      "1312|Protein purification and stability"
    ]
  ],
  "openalex": "W7223067351",
  "pdf_url": "https://europepmc.org/articles/PMC13682590?pdf=render",
  "pmcid": "PMC13682590",
  "pmid": "42903969",
  "published": "2026-10-20",
  "publisher": "Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/59b43tp9b12g4.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42903969",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42903969"
    },
    {
      "first_seen": "2026-11-07T12:23:36Z",
      "native_id": "PMC13682590",
      "source": "pmc",
      "url": "https://doi.org/10.1039/d6ra07687d"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Protein purification and stability",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Heparinization of anodized aluminum oxide (AAO) membranes for affinity separations of biocolloids.",
  "title_text": "Heparinization of anodized aluminum oxide (AAO) membranes for affinity separations of biocolloids.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:21:05Z",
  "url": "https://research.draconitic.com/paper/59b43tp9b12g4",
  "venue": "RSC advances",
  "versions": []
}