{
  "abstract": "Cancer is among the most significant diseases endangering global public health. Early detection has been demonstrated to markedly improve prognoses and increase cure rates. Hypochlorous acid (HClO) is a pivotal reactive oxygen species, frequently manifesting elevated levels in cancer cells. Herein, a highly sensitive, highly specific, and efficient HClO detection method was established based on the toehold-mediated strand displacement (TMSD). This method employs a duplex complex (TN) formed from three single-stranded DNAs to carry a fluorescent group (FAM), and introduces a hairpin DNA (HP) with a phosphorothioate (PS)-modified site for specific recognition of HClO. Under the action of HClO, the short fragment bearing FAM group is eventually released from TN, resulting in a significant increase in the system fluorescence. After optimization, the method achieves excellent HClO detection performance, with a detection range of 0.01–7 µM (LOD: 12.9 nM). Analysis of real samples, including tap water, serum and cell lysate, also confirms its good stability and accuracy. Most importantly, the method enables imaging of intracellular HClO, thereby effectively distinguishing cancer cells from normal cells. Overall, this method offers various advantages, including excellent detection performance, fast response times, ease of use, and readily available materials, making it promising for application in early diagnosis of cancer.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Clinical Laboratory, Xinxiang Central Hospital 453000 Xinxiang Henan China",
    "School of Life Sciences, Central South University 410013 Changsha Hunan China",
    "Central Laboratory, Xinxiang Central Hospital 453000 Xinxiang Henan China",
    "Department of Gynecology Oncology, Xinxiang Central Hospital 453000 Xinxiang Henan China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Qianqian Duan",
      "orcid": "0009-0004-0751-6648"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Aihua Xu"
    },
    {
      "affs": [
        2
      ],
      "name": "Ruobing Han"
    },
    {
      "affs": [
        0
      ],
      "name": "Guochao Xu"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Qianqing Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Changbei Ma",
      "orcid": "0000-0002-9973-0366"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5a1a7jf5v29b9.bib",
  "cited_by": 0,
  "doi": "10.1039/d6ra08265c",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13720905/?tool=EBI",
  "id": "5a1a7jf5v29b9",
  "issns": [
    "2046-2069"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13720905",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Chemistry"
    ],
    [
      "openalex",
      "1312|Advanced biosensing and bioanalysis techniques"
    ]
  ],
  "openalex": "W7224919623",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13720905/pdf/?tool=EBI",
  "pmcid": "PMC13720905",
  "pmid": "42964600",
  "published": "2026-10-30",
  "publisher": "Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/5a1a7jf5v29b9.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:36Z",
      "native_id": "PMC:PMC13720905",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13720905"
    },
    {
      "first_seen": "2026-11-03T12:21:42Z",
      "native_id": "PMC13720905",
      "source": "pmc",
      "url": "https://doi.org/10.1039/d6ra08265c"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Advanced biosensing and bioanalysis techniques",
      "subfield": "molecular-biology"
    }
  ],
  "title": "An efficient hypochlorous acid fluorescence imaging strategy based on toehold-mediated strand displacement for distinguishing cancer cells from normal cells",
  "title_text": "An efficient hypochlorous acid fluorescence imaging strategy based on toehold-mediated strand displacement for distinguishing cancer cells from normal cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:21:42Z",
  "url": "https://research.draconitic.com/paper/5a1a7jf5v29b9",
  "venue": "RSC advances",
  "versions": []
}