{
  "abstract": "MKK6 is a key protein kinase that regulates inflammatory response under pathogen infection by activating MAPK signal pathway. However, the function of MKK6 remained unclear within oysters. Our research recognized a novel KK6 gene from C. hongkongensis, which is named ChMKK6. The cDNA of ChMKK6 gene was 2,760 bp in length, containing an ORF of 1,032 bp encoding 344 amino acids, with a conserved S-TKc region and a DVD region. As illustrated by amino acid alignment and phylogenetic tree, ChMKK6 shared the maximal (99.0%) identity showing MKK6 of Crassostrea gigas. As indicated by tissue expression analysis, ChMKK6 possessed the maximal expression within hepatopancreas, and was markedly increased in all tissues following Vibrio Parahaemolyticus (V. Parahaemolyticus) challenge. As stated by the qRT-PCR analysis, ChMKK6 silencing significantly suppressed IL17 transcription, suggesting its involvement in innate immunity by regulating IL17-mediated inflammatory response. Moreover, ChMKK6 silencing led to the suppression of key immune-related enzyme activities (ACP, AKP, CAT, LZM, and SOD), while raising the MDA content in gill. In addition, the antimicrobial viability of hemolymph supernatant was markedly reduced by ChMKK6 silencing. These results collectively demonstrate that ChMKK6 is a key player in immunity defense of C. hongkongensis from V. Parahaemolyticus.",
  "abstract_shown": true,
  "affiliations": [
    "Fishery College of Guangdong Ocean University, Zhanjiang, China",
    "Academician Joint Laboratory of Germplasm Resource Exploitation, Utilization and Health Assessment for Aquatic Animal, Guangdong Ocean University, Zhanjiang, China",
    "Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fisheries, Guangdong Ocean University, Zhanjiang, China",
    "FangchenggangVocational and Technical College, Fangchenggang, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jiexiong Zhu"
    },
    {
      "affs": [
        1
      ],
      "name": "Guohui Shao"
    },
    {
      "affs": [
        2
      ],
      "name": "Jiayu Chen"
    },
    {
      "affs": [
        3
      ],
      "name": "Jinji Lin"
    },
    {
      "name": "Qiwen Liang"
    },
    {
      "name": "Feifei Yu"
    },
    {
      "name": "Zhiwen Wang"
    },
    {
      "name": "Baixu Lin"
    },
    {
      "name": "Yongyao Huang"
    },
    {
      "name": "Huiyan Li"
    },
    {
      "name": "Junhui Xu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5af89g9qn06k7.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0360613",
  "fulltext_url": null,
  "id": "5af89g9qn06k7",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0360613",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2403|Invertebrate Immune Response Mechanisms"
    ],
    [
      "plos",
      "/Biology and life sciences/Agriculture/Aquaculture/Oyster farming"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/RNA interference"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Signal transduction/Cell signaling/Signaling cascades/MAPK signaling cascades"
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      "/Biology and life sciences/Genetics/Gene expression"
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    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/RNA interference"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Genetic interference/RNA interference"
    ],
    [
      "plos",
      "/Biology and life sciences/Immunology/Immune response/Inflammation"
    ],
    [
      "plos",
      "/Biology and life sciences/Microbiology/Microbial control/Antimicrobials/Antibacterials"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Bacteria/Vibrio"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Animals/Invertebrates/Molluscs/Bivalves/Oysters"
    ],
    [
      "plos",
      "/Biology and life sciences/Zoology/Animals/Invertebrates/Molluscs/Bivalves/Oysters"
    ],
    [
      "plos",
      "/Medicine and health sciences/Clinical medicine/Signs and symptoms/Inflammation"
    ],
    [
      "plos",
      "/Medicine and health sciences/Immunology/Immune response/Inflammation"
    ],
    [
      "plos",
      "/Medicine and health sciences/Pharmacology/Drugs/Antimicrobials/Antibacterials"
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  "openalex": "W7222265899",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0360613&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-15",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/5af89g9qn06k7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:23Z",
      "native_id": "10.1371/journal.pone.0360613",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0360613"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
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    {
      "field": "biology",
      "origin": "plos:genetics",
      "subfield": "genetics-genomics"
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    {
      "field": "biology",
      "origin": "plos:immunology",
      "subfield": "immunology"
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  ],
  "title": "Recognition and function analysis of MKK6 in immune reaction against Vibrio Parahaemolyticus in Crassostrea hongkongensis",
  "title_text": "Recognition and function analysis of MKK6 in immune reaction against Vibrio Parahaemolyticus in Crassostrea hongkongensis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:23Z",
  "url": "https://research.draconitic.com/paper/5af89g9qn06k7",
  "venue": "PLOS One",
  "versions": []
}