{
  "abstract": "As the mesenchymal-epithelial transition factor (c-Met) is a crucial therapeutic target for multiple malignancies, we previously developed a c-Met-targeted peptide-based positron emission tomography (PET) probe, [68Ga]-Ga-SMIC-1014, derived from the Kringle 3 (K3) domain of hepatocyte growth factor (HGF). Given tumor heterogeneity, this study sought to further validate the preclinical performance of [68Ga]-Ga-SMIC-1014 and evaluate its clinical value in cancer patients. [68Ga]-Ga-SMIC-1014 was successfully synthesized with high radiochemical yield (>80%, non-decay corrected) and purity (>99%), and favorable in vitro stability in PBS and FBS. In vitro cellular uptake of the probe was positively correlated with c-Met expression levels in tumor cells, as verified by Western blot analysis. Small-animal PET and biodistribution studies demonstrated that [68Ga]-Ga-SMIC-1014 exhibited significantly higher tumor accumulation in mouse models with high c-Met expression than those with low c-Met expression (1.92 ± 0.12 vs. 0.49 ± 0.07 %ID/g at 60 min post-injection). Blocking studies with unlabeled SMIC-1014 verified the specific c-Met binding of [68Ga]-Ga-SMIC-1014 both in vitro and in vivo. The probe displayed good safety in human subjects, with physiological renal excretion and minimal-to-mild uptake in normal organs. Tumor uptake of [68Ga]-Ga-SMIC-1014 correlated well with c-Met expression levels in lung cancer patients. Collectively, [68Ga]-Ga-SMIC-1014 is safe and well tolerated in human subjects, and correlates with tumor c-Met expression, supporting its potential as a PET probe for imaging c-Met-positive tumors.",
  "abstract_shown": true,
  "affiliations": [
    "PET Center, Department of Nuclear Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510515, China.",
    "State Key Laboratory of Drug Research, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.",
    "Department of Pathology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510515, China.",
    "Department of Thoracic Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510515, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Danyi Guo"
    },
    {
      "affs": [
        0
      ],
      "name": "Yang Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Zican Feng"
    },
    {
      "affs": [
        1
      ],
      "name": "Renli Luo"
    },
    {
      "affs": [
        2
      ],
      "name": "Baozhen Zeng"
    },
    {
      "affs": [
        0
      ],
      "name": "Yinting Hu"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhenxiang Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhen Cheng",
      "orcid": "0000-0001-8177-9463"
    },
    {
      "affs": [
        3
      ],
      "name": "Dan Tian"
    },
    {
      "affs": [
        0
      ],
      "name": "Lei Jiang",
      "orcid": "0000-0002-9479-132X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5b0rzkse5g0gj.bib",
  "cited_by": 0,
  "doi": "10.1021/cbmi.6c00141",
  "fulltext_url": "https://europepmc.org/articles/PMC13715320",
  "id": "5b0rzkse5g0gj",
  "issns": [
    "2832-3637"
  ],
  "landing_url": "https://europepmc.org/article/MED/42953704",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
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      "openalex",
      "2741|Radiopharmaceutical Chemistry and Applications"
    ]
  ],
  "openalex": "W7211906668",
  "pdf_url": "https://europepmc.org/articles/PMC13715320?pdf=render",
  "pmcid": "PMC13715320",
  "pmid": "42953704",
  "published": "2026-09-07",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/5b0rzkse5g0gj.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:41Z",
      "native_id": "MED:42953704",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42953704"
    },
    {
      "first_seen": "2026-11-01T12:22:39Z",
      "native_id": "PMC13715320",
      "source": "pmc",
      "url": "https://doi.org/10.1021/cbmi.6c00141"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Radiopharmaceutical Chemistry and Applications",
      "subfield": "imaging"
    }
  ],
  "title": "Peptide-Derived [⁶⁸Ga]Ga-SMIC-1014 for c‑Met-Targeted Tumor PET Imaging: Preclinical and Pilot Clinical Studies.",
  "title_text": "Peptide-Derived [⁶⁸Ga]Ga-SMIC-1014 for c‑Met-Targeted Tumor PET Imaging: Preclinical and Pilot Clinical Studies.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:39Z",
  "url": "https://research.draconitic.com/paper/5b0rzkse5g0gj",
  "venue": "Chemical & biomedical imaging",
  "versions": []
}