{
  "abstract": "1α,25-(OH)2-D3 can promote autophagy of osteoclast precursors (OCPs), resulting in its pro-osteoclastogenic effect, which limits its anti-osteoporotic efficacy. To address the above challenges, we need to explore the targets of autophagy-enhancing effect of 1α,25-(OH)2-D3. These results showed that 1α,25-(OH)2-D3 promoted the expression of BECN1 mRNA and Beclin1 protein in OCPs under RANKL induction. Furthermore, 1α,25-(OH)2-D3 enhances p65 phosphorylation and nuclear translocation. The lentiviral-mediated silencing of both BECN1 and p65 impeded the facilitation of 1α,25-(OH)2-D3 on osteoclast differentiation and OCP autophagy. Moreover, p65 silencing blocked the enhancement of BECN1 mRNA expression and Beclin1 protein expression by 1α,25-(OH)2-D3. In conclusion, 1α,25-(OH)2-D3 enhances Beclin1-dependent autophagy in OCPs by promoting BECN1 transcription, thereby promoting osteoclast differentiation; this indicates that the therapeutic strategies for BECN1 or p65 targeted inhibition have the potential to optimize the anti-osteoporotic function of 1α,25-(OH)2-D3.",
  "abstract_shown": true,
  "affiliations": [
    "Fuzhou Second General Hospital, Department of Orthopedics, Fuzhou, China.",
    "Affiliated Jiangyin Hospital of Xuzhou Medical University, Department of Orthopedics, Jiangyin, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xi Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Hui Liu"
    },
    {
      "affs": [
        1
      ],
      "name": "Yunrong Zhu",
      "orcid": "0000-0001-6271-4226"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5cq8h78xve4pe.bib",
  "cited_by": 0,
  "doi": "10.1590/1678-4685-gmb-2025-0284",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13717831/",
  "id": "5cq8h78xve4pe",
  "issns": [
    "1415-4757",
    "1678-4685"
  ],
  "landing_url": "https://doi.org/10.1590/1678-4685-gmb-2025-0284",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Cellular, Molecular and Developmental Genetics"
    ],
    [
      "openalex",
      "1312|Bone Metabolism and Diseases"
    ]
  ],
  "openalex": "W7223405897",
  "pdf_url": "https://www.scielo.br/j/gmb/a/LT3jJRdvzYGRVhByzk99hWH/?lang=en&format=pdf",
  "pmcid": "PMC13717831",
  "pmid": "42937446",
  "published": "2026-10-23",
  "publisher": "Sociedade Brasileira de Genética",
  "ris": "https://research.draconitic.com/paper/5cq8h78xve4pe.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T12:22:05Z",
      "native_id": "PMC13717831",
      "source": "pmc",
      "url": "https://doi.org/10.1590/1678-4685-gmb-2025-0284"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Bone Metabolism and Diseases",
      "subfield": "molecular-biology"
    }
  ],
  "title": "1α,25-(OH)2-D3 promotes osteoclast-precursor autophagy and osteoclast differentiation by promoting BECN1 transcription",
  "title_text": "1α,25-(OH)2-D3 promotes osteoclast-precursor autophagy and osteoclast differentiation by promoting BECN1 transcription",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:05Z",
  "url": "https://research.draconitic.com/paper/5cq8h78xve4pe",
  "venue": "Genetics and Molecular Biology",
  "versions": []
}