{
  "abstract": "Mycobacterium tuberculosis (Mtb) is an obligate human pathogen that depends on its ability to spread from host to host to survive as a species. Knowledge of traits supporting transmission is lacking. Here, because desiccation occurs during the generation of Mtb-transmitting aerosol droplets, we exposed Mtb mounted atop a filter platform to varying degrees of humidity to characterize its transcriptomic and metabolomic responses to desiccation and rehydration. Desiccation increased levels of oxidative stress, oxidative damage and double-stranded DNA breaks, activating DNA repair responses required for survival. Expression of the transcription-coupled repair factor, mfd, also increased but this buffered the fitness cost of specific resistance-conferring mutations in rpoB, the target of the frontline drug rifampin. Silencing mfd during aerosolization specifically impaired survival of strains harbouring the most common rifampin resistance allele, S450L. This function is epidemiologically supported by whole-genome sequence analysis of 51,229 clinically circulating strains. These studies suggest that transmission-associated desiccation-induced DNA damage is a potential source of genetic diversification that can potentiate antibiotic resistance.",
  "abstract_shown": true,
  "affiliations": [
    "Weill Cornell Medical College, Department of Medicine, Division of Infectious Diseases, New York, NY USA",
    "Cornell University Meinig School of Biomedical Engineering, Ithaca, NY USA",
    "Weill Cornell Medical College, Department of Microbiology and Immunology, New York, NY USA",
    "Present Address: Biotechnology Department, National Institute of Pharmaceutical Education and Research, Sahibzada Ajit Singh Nagar, India",
    "Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ USA",
    "University of North Carolina School of Medicine, Department of Genetics, Chapel Hill, NC USA",
    "Hackensack Meridian School of Medicine, Nutley, NJ USA",
    "Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY USA",
    "Laboratory of Molecular Pathogenesis, The Rockefeller University, New York, NY USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 20,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Christopher D. Brown",
      "orcid": "0000-0003-2182-8133"
    },
    {
      "affs": [
        0
      ],
      "name": "Brendon M. Lee",
      "orcid": "0000-0001-6476-5246"
    },
    {
      "affs": [
        0
      ],
      "name": "Hannah M. Liu",
      "orcid": "0009-0001-5477-9764"
    },
    {
      "affs": [
        0
      ],
      "name": "Amy M. Wu",
      "orcid": "0009-0001-8620-7445"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrea Tellez"
    },
    {
      "affs": [
        1
      ],
      "name": "Harry Zou",
      "orcid": "0000-0003-3314-4054"
    },
    {
      "affs": [
        2
      ],
      "name": "Prabhat Ranjan Singh",
      "orcid": "0009-0004-2995-1712"
    },
    {
      "affs": [
        0
      ],
      "name": "Kohta Saito",
      "orcid": "0000-0002-2190-4127"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Saurabh Mishra",
      "orcid": "0000-0003-1590-884X"
    },
    {
      "affs": [
        0
      ],
      "name": "Megan Brown",
      "orcid": "0009-0005-0075-7171"
    },
    {
      "affs": [
        0
      ],
      "name": "Anas Saleh",
      "orcid": "0009-0008-1504-4862"
    },
    {
      "affs": [
        4
      ],
      "name": "Narineh M. Odjourian"
    },
    {
      "affs": [
        4
      ],
      "name": "Melissa Cristaldo"
    },
    {
      "affs": [
        5
      ],
      "name": "Mingyu Gan"
    },
    {
      "affs": [
        5
      ],
      "name": "Qingyun Liu",
      "orcid": "0000-0002-2284-5050"
    },
    {
      "affs": [
        4,
        6
      ],
      "name": "Martin Gengenbacher",
      "orcid": "0000-0002-0208-5920"
    },
    {
      "affs": [
        7
      ],
      "name": "Seth A. Darst",
      "orcid": "0000-0002-8241-3153"
    },
    {
      "affs": [
        8
      ],
      "name": "Elizabeth A. Campbell",
      "orcid": "0000-0002-1332-128X"
    },
    {
      "affs": [
        2
      ],
      "name": "Carl Nathan",
      "orcid": "0000-0002-7744-8896"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Kyu Y. Rhee",
      "orcid": "0000-0003-4582-2895"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5cs6zdhn4svqp.bib",
  "cited_by": 1,
  "doi": "10.1038/s41564-026-02437-w",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13716834/",
  "id": "5cs6zdhn4svqp",
  "issns": [
    "2058-5276"
  ],
  "landing_url": "https://doi.org/10.1038/s41564-026-02437-w",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2725|Tuberculosis Research and Epidemiology"
    ]
  ],
  "openalex": "W7204278471",
  "pdf_url": "https://www.nature.com/articles/s41564-026-02437-w.pdf",
  "pmcid": "PMC13716834",
  "pmid": "42649407",
  "published": "2026-08-26",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5cs6zdhn4svqp.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:24:02Z",
      "native_id": "PMC13716834",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41564-026-02437-w"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Tuberculosis Research and Epidemiology",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Desiccation promotes DNA damage and rifampin resistance in Mycobacterium tuberculosis",
  "title_text": "Desiccation promotes DNA damage and rifampin resistance in Mycobacterium tuberculosis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:21:16Z",
  "url": "https://research.draconitic.com/paper/5cs6zdhn4svqp",
  "venue": "Nature Microbiology",
  "versions": []
}