{
  "abstract": "Objective\n\nTo investigate the expression of Y1, Y3, Y4, and Y5 in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.Methods\n\nThis case-control study included 10 patients with ankylosing spondylitis and 18 healthy controls from northern and northwestern Paraná, Brazil. The diagnosis of ankylosing spondylitis was confirmed by a rheumatologist according to the 2009 and 2011 ASAS criteria. Total RNA was extracted from peripheral blood using TRIzol reagent, treated with DNase I, and reverse-transcribed into cDNA. Y RNA expression was quantified by qPCR using SYBR™ Green, and the data were analyzed using Thermo Fisher Cloud.Results\n\nOverall Y RNA expression did not differ significantly between patients and controls; however, lower ΔCt values, indicating higher Y1 and Y5 expression, were significantly associated with higher Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) scores. Pairwise comparisons showed that Y3 and Y4 were expressed at lower levels than Y1 and that Y4 expression was lower than Y3 and Y5 expression; no significant differences were observed between Y1 and Y5 or between Y3 and Y5 in either patients or controls.Conclusion\n\nThese findings suggest that Y1 and Y5 expression is associated with disease activity, although no statistically significant differences in overall Y RNA expression were observed between patients and controls.",
  "abstract_shown": true,
  "affiliations": [
    "Post-Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, PR, Brazil.",
    "Immunogenetics Laboratory, Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Guilherme Castellani",
      "orcid": "0000-0002-5474-254X"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Matheus Braga",
      "orcid": "0000-0002-6316-4200"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Aléia Harumi Uchibaba Yamanaka",
      "orcid": "0000-0001-6086-4917"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Giovana Paola Zaccarias Bemvides",
      "orcid": "0000-0002-2294-1536"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Julyane Schavaren",
      "orcid": "0009-0009-3313-5458"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Larissa Danielle Bahls Pinto",
      "orcid": "0000-0001-6469-3123"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Quirino Alves de Lima Neto",
      "orcid": "0000-0003-3313-7567"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5dbrt4xyw7t5d.bib",
  "cited_by": 0,
  "doi": "10.31744/einstein_journal/2026ao2319",
  "fulltext_url": "https://europepmc.org/articles/PMC13682767",
  "id": "5dbrt4xyw7t5d",
  "issns": [
    "1679-4508",
    "2317-6385"
  ],
  "landing_url": "https://europepmc.org/article/MED/42848639",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2745|Spondyloarthritis Studies and Treatments"
    ]
  ],
  "openalex": "W7214639385",
  "pdf_url": "https://europepmc.org/articles/PMC13682767?pdf=render",
  "pmcid": "PMC13682767",
  "pmid": "42848639",
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5dbrt4xyw7t5d.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:10Z",
      "native_id": "MED:42848639",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42848639"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2745|Spondyloarthritis Studies and Treatments",
      "subfield": "general"
    }
  ],
  "title": "Quantification of Y RNAs in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.",
  "title_text": "Quantification of Y RNAs in peripheral blood mononuclear cells from patients with ankylosing spondylitis and healthy controls.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:10Z",
  "url": "https://research.draconitic.com/paper/5dbrt4xyw7t5d",
  "venue": "Einstein (Sao Paulo, Brazil)",
  "versions": []
}