{
  "abstract": "Assembly of eukaryotic large ribosomal subunits (LSU) requires coordinated structural and compositional transitions within pre-60S particles, yet the underlying mechanisms remain poorly understood. Here, we show that the DEAD-box helicase Drs1 promotes early maturation across distinct regions of the pre-60S particle. Loss of Drs1 function causes accumulation of co-transcriptional intermediates retaining SSU processome components, indicating that Drs1 promotes timely separation of nascent LSU precursors from the small-subunit assembly pathway. Cryo-EM analyses reveal both a redistribution toward early nucleolar maturation states upon loss of Drs1, including Nsa1-deficient intermediates, and a confinement of Drs1-associated particles to states preceding stable incorporation of 25S rRNA domain III. Drs1 directly engages Erb1 through its unstructured N-terminal extension, promoting stable assembly of the Nop7-Erb1-Ytm1 module associated with domain III maturation. CRAC analysis localizes Drs1 to spatially clustered sites spanning the 5.8S and 25S rRNAs, encompassing domains I-IV. Together, these findings support a model in which Drs1 couples stabilization of assembly-factors with pre-rRNA remodeling across the pre-60S particle, thereby driving ordered early LSU maturation and the timed integration of domain III.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Molecular Biosciences, University of Graz, Austria"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "M. Thoms"
    },
    {
      "name": "S. Ayaz-Kök"
    },
    {
      "name": "K. Abe"
    },
    {
      "name": "H. Hamze"
    },
    {
      "name": "A. S. Thielen"
    },
    {
      "name": "T. Denk"
    },
    {
      "name": "B. Albert"
    },
    {
      "name": "N. Mikulic Vernik"
    },
    {
      "name": "L. Chitoiu"
    },
    {
      "name": "E. Laurent"
    },
    {
      "name": "E. Hurt"
    },
    {
      "name": "A. K. Henras"
    },
    {
      "name": "R. Beckmann"
    },
    {
      "affs": [
        0
      ],
      "name": "V. Mitterer"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5dvmv48bxn5xx.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.05.749353",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749353v1.full",
  "id": "5dvmv48bxn5xx",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749353v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1312|RNA and protein synthesis mechanisms"
    ]
  ],
  "openalex": "W7212195461",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749353v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5dvmv48bxn5xx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:42Z",
      "native_id": "10.64898/2026.09.05.749353",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749353v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA and protein synthesis mechanisms",
      "subfield": "molecular-biology"
    }
  ],
  "title": "RNA helicase Drs1 gates 25S rRNA domain III incorporation during early nucleolar pre-60S maturation",
  "title_text": "RNA helicase Drs1 gates 25S rRNA domain III incorporation during early nucleolar pre-60S maturation",
  "type": "preprint",
  "updated_at": "2026-10-30T12:20:42Z",
  "url": "https://research.draconitic.com/paper/5dvmv48bxn5xx",
  "venue": "bioRxiv",
  "versions": []
}