{
  "abstract": "Staphylococcus haemolyticus has emerged as a significant multidrug-resistant (MDR) nosocomial pathogen, yet the genomic diversity of its prophages - key drivers of bacterial evolution and horizontal gene transfer remains poorly characterized compared to S. aureus . In this study, we isolated and sequenced a novel temperate bacteriophage, IME1318_01, induced from the MDR clinical strain S. haemolyticus Sah1318. Transmission electron microscopy revealed morphology characteristic of the Siphoviridae family, featuring an isometric head and a non-contractile tail. Genomic analysis identified 61 open reading frames arranged in a functional modular architecture typical of staphylococcal prophages. To clarify its evolutionary position, we performed comprehensive phylogenomic analyses using Genome-BLAST Distance Phylogeny (GBDP) and proteomic tree reconstruction. These analyses supported the classification of IME1318_01 as a distinct species within the Azeredovirinae subfamily, forming a strongly supported monophyletic clade with Staphylococcus phage StB12. Biological assays indicated a latent period of approximately 20 min and a burst size of 56 PFU per cell, confirming efficient replication dynamics upon induction. These findings expand our understanding of the 'viral dark matter' in coagulase-negative staphylococci and offer valuable clues regarding the genetic mobility and host interactions of phages within antibiotic-resistant reservoirs.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Respiratory and Critical Care Medicine, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China",
    "Outpatient Department, Jingnan Medical Area, Chinese PLA General Hospital, Beijing, 100161, China",
    "Department of Respiratory and Critical Care Medicine, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China; Corresponding author at: Department of Respiratory and Critical Care Medicine, Fuyang Hospital of Anhui Medical University, Fuyang 236000, China."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Qiang Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Lijuan Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Can Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5e4q511wy0rm7.bib",
  "cited_by": 0,
  "doi": "10.1016/j.microb.2026.100820",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2950194626001664",
  "id": "5e4q511wy0rm7",
  "issns": [
    "2950-1946"
  ],
  "landing_url": "https://doi.org/10.1016/j.microb.2026.100820",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QR1-502"
    ],
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7223135842",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/5e4q511wy0rm7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:44:35Z",
      "native_id": "b755ef7f02f44be3a18ed267e4eeca59",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.microb.2026.100820"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2303|Bacteriophages and microbial interactions",
      "subfield": "ecology"
    }
  ],
  "title": "Characterization and genome analysis of IME1318_01, a novel temperate bacteriophage induced from",
  "title_text": "Characterization and genome analysis of IME1318_01, a novel temperate bacteriophage induced from",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:44:35Z",
  "url": "https://research.draconitic.com/paper/5e4q511wy0rm7",
  "venue": "The Microbe",
  "versions": []
}