{
  "abstract": "The continuous evolution of SARS-CoV-2 necessitates broadly neutralizing antibodies for robust therapeutics. In this study, we isolated 1824 single B cells from a COVID-19 convalescent donor and obtained 91 pairs of nucleotide sequences potentially encoding VH/VL domains. From this library, we generated and evaluated a panel of 38 recombinant monoclonal antibodies. Among this panel, we identified DEV_K57 as a broadly neutralizing human IgG1 antibody targeting the SARS-CoV-2 RBD. DEV_K57 exhibited in vitro neutralization breadth against diverse variants, demonstrating subnanomolar IC50 values. This in vitro efficacy was translated in vivo. In a Syrian hamster model of SARS-CoV-2 Delta infection, DEV_K57 significantly reduced viral RNA and infectious titers in both the upper and lower respiratory tracts, conferring a favorable safety profile with minimal histopathological damage. To elucidate its mechanism, we performed comprehensive biophysical and structural analyses. Biolayer interferometry revealed a high picomolar binding affinity to the RBD Delta. Furthermore, size-exclusion chromatography coupled with small-angle X-ray scattering and computational modeling uncovered a unique, stable dimeric architecture of the DEV_K57 Fab-RBD complex in solution, with a binding interface distinct from previously characterized structures. These findings underscore the value of rapid antibody isolation for pandemic preparedness against future emerging biological threats.",
  "abstract_shown": true,
  "affiliations": [
    "State Research Center of Virology and Biotechnology “Vector”, 630559 Kol’tsovo, Russia",
    "Moscow Center for Advanced Studies, 123592 Moscow, Russia"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Elina V. Sinitsina"
    },
    {
      "affs": [
        0
      ],
      "name": "Ilnaz R. Imatdinov"
    },
    {
      "affs": [
        0
      ],
      "name": "Vitaliy Y. Tuyrin"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrey V. Shipovalov",
      "orcid": "0000-0003-1201-8307"
    },
    {
      "affs": [
        0
      ],
      "name": "Anna V. Zaykovskaya"
    },
    {
      "affs": [
        0
      ],
      "name": "Irina A. Osinkina",
      "orcid": "0009-0009-6942-0840"
    },
    {
      "affs": [
        0
      ],
      "name": "Alexey O. Yanshin"
    },
    {
      "affs": [
        0
      ],
      "name": "Nikita S. Radchenko",
      "orcid": "0009-0002-9524-9062"
    },
    {
      "affs": [
        1
      ],
      "name": "Yury L. Ryzhykau",
      "orcid": "0000-0003-3719-1464"
    },
    {
      "affs": [
        1
      ],
      "name": "Vsevolod V. Sudarev",
      "orcid": "0000-0001-6681-0442"
    },
    {
      "affs": [
        1
      ],
      "name": "Daria D. Kuklina"
    },
    {
      "affs": [
        0
      ],
      "name": "Galina S. Onkhonova"
    },
    {
      "affs": [
        0
      ],
      "name": "Elena K. Ivleva"
    },
    {
      "affs": [
        0
      ],
      "name": "Alexander P. Agafonov",
      "orcid": "0000-0003-2577-0434"
    },
    {
      "affs": [
        0
      ],
      "name": "Anastasia V. Gladysheva",
      "orcid": "0000-0002-7396-3954"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5ekp1zy8kydy3.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219445",
  "fulltext_url": null,
  "id": "5ekp1zy8kydy3",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9445",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2725|SARS-CoV-2 and COVID-19 Research"
    ]
  ],
  "openalex": "W7223402876",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-23",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/5ekp1zy8kydy3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:57Z",
      "native_id": "10.3390/ijms27219445",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9445"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|SARS-CoV-2 and COVID-19 Research",
      "subfield": "infectious-disease"
    }
  ],
  "title": "The Broadly Neutralizing DEV_K57 Antibody, Isolated from a Convalescent Patient, in Complex with the SARS-CoV-2 RBD",
  "title_text": "The Broadly Neutralizing DEV_K57 Antibody, Isolated from a Convalescent Patient, in Complex with the SARS-CoV-2 RBD",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:57Z",
  "url": "https://research.draconitic.com/paper/5ekp1zy8kydy3",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}