{
  "abstract": "Invasive placental cells, extravillous trophoblasts (EVT), remodel maternal vasculature to meet metabolic needs of the growing fetus. Dysregulation of spiral artery remodeling (SAR) is a characteristic of preeclampsia (PE), a severe pregnancy-related condition. We developed a modular new approach methodology (NAM) of human endometrial-specific vascular networks in a polystyrene (PS) microfluidic chip to recapitulate the intricate endometrial vascular microenvironment to investigate SAR. We leveraged the self-assembly of human endometrial microvascular endothelial cells (HEMEC) and normal human lung fibroblasts (NHLF) to successfully develop patent endometrial vascular networks after 3 days of culture. Exogenous proangiogenic factors significantly improved vascular density while maintaining intrinsic endometrial vascular properties. Treatments of menstrual cycle phase-specific hormones differentially affected endometrial vascular density, diameter, permeability, and endogenous secretions of proangiogenic proteins. We evaluated integration potential of EVT cell lines HTR8/SVneo and JEG-3. We measured decreased barrier function and observed increased presence of apoptotic HEMECs after 3 days of tri-culture with JEG-3 cells, whereas HTR8/SVneo-induced apoptosis remained statistically unchanged across days 1 and 3 of integration. Moreover, we confirm HTR8/SVneo and JEG-3 cells differentially interact with HEMEC vasculature and the surrounding perivascular niche. These studies demonstrate the utility of our model to study endometrial vascularization and EVT remodeling.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, Massachusetts, USA. jywong@bu.edu.",
    "Seoul National University, Gwanak-gu, Republic of Korea."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sebastian Naranjo",
      "orcid": "0000-0001-7740-5506"
    },
    {
      "affs": [
        0
      ],
      "name": "Madison E Fletcher"
    },
    {
      "affs": [
        1
      ],
      "name": "Noo Li Jeon",
      "orcid": "0000-0003-0490-3592"
    },
    {
      "affs": [
        0
      ],
      "name": "Joyce Y Wong",
      "orcid": "0000-0002-3526-6381"
    },
    {
      "affs": [
        0
      ],
      "name": "Catherine Klapperich",
      "orcid": "0000-0001-6103-849X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5f699qyh2q3sg.bib",
  "cited_by": 0,
  "doi": "10.1039/d6lc00513f",
  "fulltext_url": "https://europepmc.org/articles/PMC13651457",
  "id": "5f699qyh2q3sg",
  "issns": [
    "1473-0197",
    "1473-0189"
  ],
  "landing_url": "https://europepmc.org/article/MED/42852710",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2729|Pregnancy and preeclampsia studies"
    ]
  ],
  "openalex": "W7215097372",
  "pdf_url": "https://pubs.rsc.org/lc/article-pdf/doi/10.1039/D6LC00513F/14813558/d6lc00513f.pdf",
  "pmcid": "PMC13651457",
  "pmid": "42852710",
  "published": "2026-10-09",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5f699qyh2q3sg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42852710",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42852710"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2729|Pregnancy and preeclampsia studies",
      "subfield": "reproductive-child"
    }
  ],
  "title": "A perfusable first-generation endometrial microvascular model to study human spiral artery remodeling in pregnancy.",
  "title_text": "A perfusable first-generation endometrial microvascular model to study human spiral artery remodeling in pregnancy.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/5f699qyh2q3sg",
  "venue": "Lab on a chip",
  "versions": []
}