{
  "abstract": "β-(1,3)-glucans are important for the integrity of the fungal cell wall and the interaction of fungi with the (a)biotic environment. For instance, the β-(1,3)(1,6)-glucan schizophyllan protects against thermal stress, starvation, and competition. Agaricomycetes like Schizophyllum commune have two β-(1,3)-glucan synthase (bgs) genes. Here, deletion constructs for the bgs1 and bgs2 genes were introduced in S. commune . A Δbgs2 strain was not obtained. Inactivation of bgs1 resulted in an approximately twofold lower production of water-soluble polysaccharide with FTIR characteristics consistent with schizophyllan. In addition, solid state NMR showed a relative 5-fold decrease in β-(1,3)-glucan / β-(1,3)(1,6)-glucan (schizophyllan) levels in the rigid part of the cell wall of the deletion strain, which was accompanied by a relative 3.3-fold and 2.7-fold increase in chitin and α-(1–3)-glucan, respectively. These changes in cell wall composition were accompanied by reduced growth, impaired fruiting body formation, and absence of spore production. Together, these data show for the first time the impact of a deletion of a β-(1,3)-glucan synthase gene in a mushroom-forming fungus on cell wall composition, growth, and fruiting body development.",
  "abstract_shown": true,
  "affiliations": [
    "Microbiology, Department of Biology, Utrecht University, Padualaan 8, Utrecht, 3584 CH, the Netherlands",
    "NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, Utrecht, 3584 CH, the Netherlands",
    "Microbiology, Department of Biology, Utrecht University, Padualaan 8, Utrecht, 3584 CH, the Netherlands; Corresponding author at: Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ella M. Schunselaar"
    },
    {
      "affs": [
        0
      ],
      "name": "Fleur E.L. Kleijburg"
    },
    {
      "affs": [
        1
      ],
      "name": "Adil A. Safeer"
    },
    {
      "affs": [
        1
      ],
      "name": "Ajit K. Bishoyi"
    },
    {
      "affs": [
        0
      ],
      "name": "Sophie J.L. van Velzen"
    },
    {
      "affs": [
        0
      ],
      "name": "Peter Jan Vonk"
    },
    {
      "affs": [
        0
      ],
      "name": "Luis G. Lugones"
    },
    {
      "affs": [
        1
      ],
      "name": "Marc Baldus"
    },
    {
      "affs": [
        2
      ],
      "name": "Han A.B. Wösten"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5f7jzyppvwcaz.bib",
  "cited_by": 0,
  "doi": "10.1016/j.tcsw.2026.100183",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2468233026000162",
  "id": "5f7jzyppvwcaz",
  "issns": [
    "2468-2330"
  ],
  "landing_url": "https://doi.org/10.1016/j.tcsw.2026.100183",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Paper"
    ],
    [
      "lcc",
      "QH573-671"
    ],
    [
      "openalex",
      "1312|Fungal and yeast genetics research"
    ]
  ],
  "openalex": "W7222558849",
  "pdf_url": "https://europepmc.org/articles/PMC13718416?pdf=render",
  "pmcid": null,
  "pmid": "42960147",
  "published": "2026-10-16",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/5f7jzyppvwcaz.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:44:30Z",
      "native_id": "4b9fa011fefa4b41a91741a567159bf5",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.tcsw.2026.100183"
    },
    {
      "first_seen": "2026-11-01T00:20:52Z",
      "native_id": "MED:42960147",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42960147"
    },
    {
      "first_seen": "2026-11-02T12:22:27Z",
      "native_id": "PMC13718416",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.tcsw.2026.100183"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Fungal and yeast genetics research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Deletion of the predicted β-(1,3)-glucan synthase gene",
  "title_text": "Deletion of the predicted β-(1,3)-glucan synthase gene",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:27Z",
  "url": "https://research.draconitic.com/paper/5f7jzyppvwcaz",
  "venue": "The Cell Surface",
  "versions": []
}