{
  "abstract": "Serine/arginine-rich splicing factor 1 (SRSF1) regulates alternative splicing and acts as a proto-oncogene, but its role in transcription remains poorly defined. We combined time-course RNA-seq with experimental validation to define SRSF1-dependent transcriptional and splicing programs. We validated 67 of 143 alternative-splicing events selected for experimental analysis. Among these, 44 were not annotated in GENCODE, including 15 containing previously unannotated exons. Thirty-five events showed concordant regulation in HEK293 and breast and prostate cancer cell lines, and 17 events in 16 genes affected p53 regulation or p53-dependent functions. SRSF1 overexpression reduced total p53, increased basal Ser15 phosphorylation, and increased apoptosis under basal and cisplatin-treated conditions but not following doxorubicin treatment. SRSF1 also increased transcription of 28 genes, 18 of which showed concordant regulation across the same cellular models. Mechanistic studies linked SRSF1-dependent transcriptional activation to P-TEFb mobilization from the 7SK snRNP, which controls P-TEFb availability for RNA polymerase II elongation. These findings identify distinct but concurrent SRSF1-regulated programs: transcriptional activation of genes associated with cellular signaling and organization and alternative splicing modulating genome-maintenance and p53-regulatory genes.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Sean Paz"
    },
    {
      "name": "Elena Bisotto"
    },
    {
      "name": "Massimo Caputi",
      "orcid": "0000-0002-4926-9700"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5fz5k7d3xft9s.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.16.759576",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759576v1.full",
  "id": "5fz5k7d3xft9s",
  "issns": [
    "2692-8205"
  ],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759576v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ],
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ]
  ],
  "openalex": "W7222964432",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759576v1.full.pdf",
  "pmcid": "PMC13733691",
  "pmid": "42990148",
  "published": "2026-10-16",
  "publisher": "Cold Spring Harbor Laboratory Preprints",
  "ris": "https://research.draconitic.com/paper/5fz5k7d3xft9s.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:38Z",
      "native_id": "10.64898/2026.10.16.759576",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.16.759576v2"
    },
    {
      "first_seen": "2026-11-08T12:23:24Z",
      "native_id": "PMC13733691",
      "source": "pmc",
      "url": "https://doi.org/10.64898/2026.10.16.759576"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "SRSF1 coordinates transcription and alternative splicing to modulate p53 and genome-maintenance",
  "title_text": "SRSF1 coordinates transcription and alternative splicing to modulate p53 and genome-maintenance",
  "type": "preprint",
  "updated_at": "2026-11-08T12:23:24Z",
  "url": "https://research.draconitic.com/paper/5fz5k7d3xft9s",
  "venue": "bioRxiv",
  "versions": []
}