{
  "abstract": "Using CLAP methodology, Guo et al. recently concluded that PRC2 is not an RNA binding protein (RBP). They suggest that prior findings were CLIP artifacts and argue against RNA's direct role in PRC2 regulation. Here, we re-analyze their raw datasets and reach contrary conclusions. Through an independent computational pipeline, we observe significant PRC2 enrichment throughout the transcriptome, including XIST. Applying the authors' published computational pipeline also reaffirms PRC2 as an RBP. Detailed investigation of the authors' pipeline reveals several unconventional practices. First, Guo et al. retained reads from foreign species and other unmappable reads to obtain a normalization factor. Second, they selectively removed read duplicates from the mappable fraction, while retaining them in the unmappable fraction. Finally, the authors applied an arbitrary cutoff for enrichment values in XIST. Their pipeline thereby inflated PRC2's background reads and suppressed mappable signals, creating the impression that PRC2 is not a robust RBP.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Genetics, Harvard Medical School, Boston, MA, USA",
    "Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA",
    "Institute of Nanotechnology and Advanced Materials, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "YongWoo Lee"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Priyojit Das",
      "orcid": "0000-0002-6774-6718"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Barry Kesner"
    },
    {
      "affs": [
        2
      ],
      "name": "Michael Rosenberg"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Roy Blum",
      "orcid": "0000-0002-3987-7198"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jeannie T. Lee",
      "orcid": "0000-0001-7786-8850"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5g6mqnmb2dnqn.bib",
  "cited_by": 16,
  "doi": "10.1016/j.patter.2026.101641",
  "fulltext_url": null,
  "id": "5g6mqnmb2dnqn",
  "issns": [
    "2666-3899"
  ],
  "landing_url": "https://doi.org/10.1016/j.patter.2026.101641",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ]
  ],
  "openalex": "W7203867602",
  "pdf_url": "https://www.cell.com/patterns/pdf/S2666-3899(26)00150-9.pdf",
  "pmcid": "PMC11429800",
  "pmid": "39345380",
  "published": "2026-08-01",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/5g6mqnmb2dnqn.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T13:21:05Z",
      "native_id": "W7203867602",
      "source": "openalex",
      "url": "https://doi.org/10.1016/j.patter.2026.101641"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA Research and Splicing",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Re-analysis of CLAP supports prior evidence of PRC2 as an RNA-binding protein",
  "title_text": "Re-analysis of CLAP supports prior evidence of PRC2 as an RNA-binding protein",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:05Z",
  "url": "https://research.draconitic.com/paper/5g6mqnmb2dnqn",
  "venue": "Patterns",
  "versions": []
}