{
  "abstract": "The 5'-untranslated region (5'-UTR) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly structured and comprises five conserved stem-loops that serve as cis-acting regulatory elements. During infection, host proteins are recruited to these elements and regulate multiple steps during the viral life cycle. Stem-loop 5 (SL5) is highly conserved among different alpha- and betacoronaviruses and includes the start codon of non-structural protein 1 (nsp1). However, the function of SL5 in viral replication and its interacting proteins remain to be determined. Here, we identified heterogeneous nuclear ribonucleoprotein M (HNRNPM) as a host protein functionally interacting with SL5. HNRNPM translocates into the cytoplasm during SARS-CoV-2 infection and promotes protein synthesis from the viral 5'-UTR. Mutations in the lower stem of SL5 impair HNRNPM binding and reduce protein synthesis. Notably, the interaction between HNRNPM and SL5 is conserved among different alpha- and betacoronaviruses. Our findings indicate that HNRNPM is an important host factor that regulates viral translation to promote SARS-CoV-2 replication.",
  "abstract_shown": true,
  "affiliations": [
    "Archives School Marburg"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "J. Golagha"
    },
    {
      "name": "M. Wieja"
    },
    {
      "name": "N. Biedenkopf"
    },
    {
      "affs": [
        0
      ],
      "name": "J. E. Weigand"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5h71zan1m2qfs.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.05.756635",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756635v1.full",
  "id": "5h71zan1m2qfs",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756635v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "2725|SARS-CoV-2 and COVID-19 Research"
    ]
  ],
  "openalex": "W7220705517",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756635v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/5h71zan1m2qfs.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:08Z",
      "native_id": "10.64898/2026.10.05.756635",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.05.756635v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|SARS-CoV-2 and COVID-19 Research",
      "subfield": "infectious-disease"
    }
  ],
  "title": "HNRNPM promotes protein synthesis via an interaction with stem-loop 5 in the SARS-CoV-2 5'-UTR",
  "title_text": "HNRNPM promotes protein synthesis via an interaction with stem-loop 5 in the SARS-CoV-2 5'-UTR",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:08Z",
  "url": "https://research.draconitic.com/paper/5h71zan1m2qfs",
  "venue": "bioRxiv",
  "versions": []
}