{
  "abstract": "A novel lytic bacteriophage, designated vB_ValS-L9, infecting the potentially pathogenic bacterium Vibrio alginolyticus L4, was isolated from a coastal aquaculture area in Beihai, China. Transmission electron microscopy revealed that vB_ValS-L9 possesses an icosahedral head (56.50 ± 4.19 nm in diameter) and a long tail (143.03 ± 5.32 nm in length), exhibiting a typical siphovirus-like morphology within the class Caudoviricetes. The phage demonstrated stability across various temperatures, pH levels, and salinities. Genomic sequencing showed that vB_ValS-L9 has a circular double-stranded DNA genome of 44,025 bp with a G + C content of 43.56% and 61 predicted ORFs. No tRNA, virulence, or antibiotic resistance genes were identified. Genomic and phylogenetic analyses, including ANI, VICTOR, VIRIDIC, and conserved protein phylogenies, identified vB_ValS-L9 as a novel species that, together with Vibrio phages Artemius and VapS30, forms a new genus in the class Caudoviricetes, for which the name Artemiovirus is proposed. Furthermore, in vivo trials using juvenile pearl oysters (Pinctada martensii) revealed that vB_ValS-L9 provides substantial prophylactic and therapeutic protection against V. alginolyticus L4 infection, especially when the phage was administered before bacterial challenge. Collectively, these findings suggest that vB_ValS-L9 shows potential as a safe, environmentally robust, and promising biocontrol candidate for managing vibriosis in aquaculture.",
  "abstract_shown": true,
  "affiliations": [
    "Guangxi Laboratory of Oceanography, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China",
    "Key Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China",
    "Laboratory of Viral & Microbial Diversity, Ocean University of China, Qingdao 266003, China",
    "College of Oceanography and Ecological Science, Shanghai Ocean University, Shanghai 201306, China",
    "College of Oceanography, Beibu Gulf University, Qinzhou 535011, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Xuyang Chen"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xuejing Li"
    },
    {
      "affs": [
        2
      ],
      "name": "Yiyang Zhu"
    },
    {
      "affs": [
        2
      ],
      "name": "Yantao Liang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xing Ren"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Shumeng Zhang"
    },
    {
      "affs": [
        3
      ],
      "name": "Junyi Tao"
    },
    {
      "affs": [
        4
      ],
      "name": "Kailiang Zou"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Longfei Lu",
      "orcid": "0000-0001-5405-6681"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5hyes4968ypwg.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27209385",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705570/?tool=EBI",
  "id": "5hyes4968ypwg",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/9385",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7214157566",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705570/pdf/?tool=EBI",
  "pmcid": "PMC13705570",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/5hyes4968ypwg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:57Z",
      "native_id": "10.3390/ijms27209385",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/9385"
    },
    {
      "first_seen": "2026-10-30T12:21:45Z",
      "native_id": "PMC:PMC13705570",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13705570"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2303|Bacteriophages and microbial interactions",
      "subfield": "ecology"
    }
  ],
  "title": "Characterization and Genome Analysis of a Novel Vibrio Alginolyticus Phage and Its Biocontrol Efficacy in Pearl Oyster Aquaculture",
  "title_text": "Characterization and Genome Analysis of a Novel Vibrio Alginolyticus Phage and Its Biocontrol Efficacy in Pearl Oyster Aquaculture",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:45Z",
  "url": "https://research.draconitic.com/paper/5hyes4968ypwg",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}