{
  "abstract": "Background\n\nThe objective of this study was to analyze the tuberculosis(TB) positivity rate of formalin-fixed paraffin-embedded (FFPE) tissue samples, to explore the difference between the positivity rates of pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB), and to compare the value of fluorescent probe-based polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis DNA (TB-DNA) and modified Ziehl-Neelsen acid-fast staining (Z-N staining) in the diagnosis of tuberculosis.Methods\n\nA total of 491 patients with histopathological suspected TB admitted to Hainan General Hospital from October 2022 to March 2024 were enrolled. FFPE tissue samples were collected from all participants and examined by fluorescence probe PCR for TB-DNA, acid‑fast staining, and hematoxylin-eosin (H&E) staining. The positive detection rates of TB among different assays were compared. With fluorescent probe PCR results serving as the reference standard, the diagnostic performance of modified Z-N staining was further evaluated. Count data were analyzed using the chi-square test, and a fourfold table was applied to assess the diagnostic efficacy.Results\n\nTB was detected by PCR in 278 cases and by modified Z-N staining in 89 cases. analysis revealed that age, modified Z-N staining results, musculoskeletal tuberculosis (MSTB), and intra-abdominal tuberculosis were significantly associated with TB-PCR results (P < 0.05). The median age was 52 years (interquartile range [IQR]: 37-65 years). Among FFPE tissue samples, the highest proportion of PCR positivity was found in the young group (18-44 years) at 65.97%, while the highest proportion of PCR negativity was seen in the middle-aged group (45-59 years) at 46.84%. The most common sites of EPTB involvement were the pleura, head and neck, and musculoskeletal system, with an overall proportion of 36.46% (179/491). modified Z-N staining was closely correlated with TB-PCR outcomes in both PTB and EPTB subgroups.Conclusions\n\nThe use of PCR detection of TB-DNA is more effective than modified Z-N staining for the diagnosis of PTB and EPTB. It is recommended that FFPE tissue samples are prioritised for screening for Mycobacterium tuberculosis by PCR to speed up the diagnosis of TB.Clinical trial\n\nNot applicable.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pathology, Hainan General Hospital, Affiliated Hainan Hospital of Hainan Medical University, 19 Xiuhua Road, Haikou, Hainan Province, 570311, China.",
    "Medical Laboratory Center, Hainan General Hospital, Affiliated Hainan Hospital of Hainan Medical University, Hainan Province, Haikou, 570311, China.",
    "Department of Pathology, Hainan General Hospital, Affiliated Hainan Hospital of Hainan Medical University, 19 Xiuhua Road, Haikou, Hainan Province, 570311, China. wangqugans@163.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yu Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Sha-Sha Hu"
    },
    {
      "affs": [
        0
      ],
      "name": "Shan-Yong Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Shu Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Bo Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5mt7nmnqa0nfg.bib",
  "cited_by": 0,
  "doi": "10.1186/s12879-026-14175-9",
  "fulltext_url": "https://europepmc.org/articles/PMC13729449",
  "id": "5mt7nmnqa0nfg",
  "issns": [
    "1471-2334"
  ],
  "landing_url": "https://europepmc.org/article/MED/42972510",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "2725|Tuberculosis Research and Epidemiology"
    ]
  ],
  "openalex": "W7172489155",
  "pdf_url": "https://europepmc.org/articles/PMC13729449?pdf=render",
  "pmcid": "PMC13729449",
  "pmid": "42972510",
  "published": "2026-08-05",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/5mt7nmnqa0nfg.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:44Z",
      "native_id": "MED:42972510",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42972510"
    },
    {
      "first_seen": "2026-11-07T12:22:12Z",
      "native_id": "PMC13729449",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s12879-026-14175-9"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Tuberculosis Research and Epidemiology",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Analysis of the prevalence of positivity for tuberculosis infection in formalin-fixed paraffin-embedded tissue.",
  "title_text": "Analysis of the prevalence of positivity for tuberculosis infection in formalin-fixed paraffin-embedded tissue.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:22:12Z",
  "url": "https://research.draconitic.com/paper/5mt7nmnqa0nfg",
  "venue": "BMC infectious diseases",
  "versions": []
}