{
  "abstract": "As machine learning (ML) becomes increasingly important in protein engineering, synthetic biology, and bioprocess optimization, the lack of consistent, complete, and standardized experimental reporting remains a major barrier to building effective models. EXPERT (EXpression and Purification Experimental Reporting Template) defines a structured framework for capturing protein expression and purification data across the community, to increase reproducibility across laboratories and support downstream ML applications. We propose here the essential data that should be recorded during expression and purification workflows by both novice and experienced protein production scientists. These recommendations are informed by expert contributions from across academia and industry. The reporting requirements were distilled to a focused set of parameters deemed sufficient for workflow reproducibility and constructing robust ML models aimed at improving protein production. By deploying these guidelines, we hope to enable the collection of larger data sets to support future model building.",
  "abstract_shown": true,
  "affiliations": [
    "School of Biosciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom",
    "European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, United Kingdom",
    "European Molecular Biology Laboratory, Protein Expression and Purification Core Facility, Heidelberg, Germany",
    "Institut Curie, CurieCoreTech, PSL University, Recombinant Proteins Core Facility, CNRS, Paris, France",
    "Structural Genomics Consortium, University of Toronto and University Health Network, Toronto, Ontario, Canada",
    "Membrane Protein Laboratory, Research Complex at Harwell, Diamond Light Source, Didcot, United Kingdom",
    "Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Technology Platform for Protein Production & Characterization, Berlin, Germany",
    "Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), Potsdam, Germany",
    "Laboratory of Environmental and Life Science, University of Nova Gorica, Nova Gorica, Slovenia",
    "Department of Protein Science, SciLifeLab, KTH-Royal Institute of Technology, Stockholm, Sweden",
    "Max Planck Institute of Molecular Cell Biology and Genetics, Protein Biochemistry facility, Dresden, Germany",
    "Biomodal, Chesterford Research Park, Cambridge, United Kingdom",
    "Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark",
    "Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain",
    "Kemp Proteins, LLC, Frederick, Maryland, USA",
    "Mammalian Protein Expression Core Facility and Protein Production Sweden, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden",
    "Pfizer Research and Development, Cambridge, USA",
    "Vienna BioCenter Core Facilities GmbH, Vienna, Austria",
    "University College London, Department of Computer Science and Department of Structural and Molecular Biology, London, United Kingdom",
    "Generative Biology Institute, Ellison Institute of Technology Oxford, The Oxford Science Park, Oxford, United Kingdom",
    "School of Informatics, The University of Edinburgh, Edinburgh, United Kingdom",
    "School of Biological Sciences, The University of Edinburgh, Edinburgh, United Kingdom",
    "Septerna, South San Francisco, USA",
    "Lund Protein Production Platform & Protein Production Sweden & Science for Life Laboratory, Lund University, Lund, Sweden",
    "Institute of Biophysical Chemistry, Goethe-University Frankfurt, Frankfurt/Main, Germany",
    "i3s - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal",
    "IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal",
    "Novartis, Biomedical Research, Cambridge, Massachusetts, USA",
    "Max Planck-Bristol Centre for Minimal Biology, Schools of Chemistry and Biochemistry, University of Bristol, Bristol, United Kingdom",
    "Lila Sciences, Cambridge, USA",
    "Institute of Biotechnology of the Czech Academy of Sciences, Laboratory of Structure and Function of Biomolecules, Vestec, Czech Republic",
    "School of Pharmacy and Structural Genomics Consortium, UCL, London, United Kingdom",
    "Max Planck Institute of Biochemistry, Protein Production and Structural Validation Facility, Martinsried, Germany",
    "Cleveland Clinic Center for Therapeutics Discovery, Cleveland Clinic Research, Cleveland, USA",
    "BOKU University, Department of Biotechnology and Food Science (BTLW), Institute of Bioprocess Science and Engineering (IBSE), Vienna, Austria",
    "Helmholtz Centre for Infection Research, Department of Molecular Structural Biology (MOSB), Braunschweig, Germany",
    "VIB Protein Core, VIB Technologies, VIB, Ghent, Belgium",
    "Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium",
    "Department of Chemistry, Kavli Institute of Nanoscience Discovery, University of Oxford, Oxford, United Kingdom",
    "Protein Production and Structure Characterization Core Facility (PTPSP), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 86,
  "authors": [
    {
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        0
      ],
      "name": "Christopher D.O. Cooper"
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        1
      ],
      "name": "Evgeny Tankhilevich"
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        2
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      "name": "Kim Remans"
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        3
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      "name": "Ahmed El Marjou"
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        4
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      "name": "Aled M. Edwards"
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        1
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      "name": "Andrew R. Leach"
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        5
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      "name": "Andrew Quigley"
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      "name": "Anja Schütz"
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      "name": "Anne Zemella"
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        8
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      "name": "Ario de Marco"
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      "name": "Åsa Sivertsson"
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      "name": "Barbara Borgonovo"
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      "name": "Cecilia Wickstrand"
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      "name": "Dafydd R. Owen"
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      "name": "David Drechsel"
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        18
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      "name": "David Jones"
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        19,
        20,
        21
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      "name": "Diego A. Oyarzún"
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      "name": "Dominic Esposito"
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        10
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      "name": "Eric R. Geertsma"
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        23
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      "name": "Ewa Krupinska"
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        24
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      "name": "Frank Bernhard"
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        25,
        26
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      "name": "Frederico Ferreira-da-Silva"
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    {
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        9
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      "name": "Hanna Tegel"
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        27
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      "name": "Ian Hunt"
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        28
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      "name": "Imre Berger"
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        29
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      "name": "James Love"
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        30
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      "name": "Jan Dohnálek"
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        31
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      "name": "Jelena Thies"
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    {
      "affs": [
        32
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      "name": "Jerome Basquin"
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    {
      "affs": [
        33
      ],
      "name": "Jesse Coker"
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    {
      "affs": [
        34
      ],
      "name": "Johannes Buyel"
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        35
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      "name": "Joop van den Heuvel"
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        36,
        37
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      "name": "Jurgen Haustraete"
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      "affs": [
        38
      ],
      "name": "Katharina L. Dürr"
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    {
      "affs": [
        39
      ],
      "name": "Kelvin Lau"
    },
    {
      "name": "Kristina Hedfalk"
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    {
      "affs": [
        4
      ],
      "name": "Kristof Bozovicar"
    },
    {
      "affs": [
        4
      ],
      "name": "Lihua Liu"
    },
    {
      "name": "Lisa Bamber"
    },
    {
      "name": "Luigi Angelo Scietti"
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    {
      "affs": [
        15
      ],
      "name": "Malin Bäckström"
    },
    {
      "name": "Maren Schubert"
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    {
      "name": "Mark Elvin"
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    {
      "name": "Martin Pelosse"
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    {
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        31
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      "name": "Matthew H. Todd"
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    {
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        4
      ],
      "name": "Matthieu Schapira"
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    {
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        30
      ],
      "name": "Miroslava Alblová"
    },
    {
      "name": "Nicholas S. Berrow"
    },
    {
      "name": "Nikolay Marinchev Dobrev"
    },
    {
      "name": "Oleg Brodsky"
    },
    {
      "name": "Ondřej Vaněk"
    },
    {
      "name": "Opher Gileadi"
    },
    {
      "name": "Pam Dossang"
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    {
      "name": "Paola Storici"
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    {
      "name": "Patrick Celie"
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    {
      "name": "Paul Wan"
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  ],
  "bibtex": "https://research.draconitic.com/paper/5n0bzt0qpsw95.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jbc.2026.113610",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13738122/",
  "id": "5n0bzt0qpsw95",
  "issns": [
    "0021-9258",
    "1083-351X"
  ],
  "landing_url": "https://doi.org/10.1016/j.jbc.2026.113610",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Methods and Resources"
    ],
    [
      "openalex",
      "1312|Protein purification and stability"
    ]
  ],
  "openalex": "W7214700973",
  "pdf_url": null,
  "pmcid": "PMC13738122",
  "pmid": "42810683",
  "published": "2026-09-29",
  "publisher": "American Society for Biochemistry and Molecular Biology",
  "ris": "https://research.draconitic.com/paper/5n0bzt0qpsw95.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:23:10Z",
      "native_id": "PMC13738122",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.jbc.2026.113610"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Protein purification and stability",
      "subfield": "molecular-biology"
    }
  ],
  "title": "EXPERT: A practical and forward-looking guide to capturing protein expression and purification metadata to develop machine learning models",
  "title_text": "EXPERT: A practical and forward-looking guide to capturing protein expression and purification metadata to develop machine learning models",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:23:10Z",
  "url": "https://research.draconitic.com/paper/5n0bzt0qpsw95",
  "venue": "The Journal of Biological Chemistry",
  "versions": []
}