{
  "abstract": "Pathogen survival relies on their ability to adapt to changing environments, yet how intracellular bacteria tolerate cell-autonomous immunity remains unclear. Septins assemble into cage-like structures around cytoplasmic Shigella targeted by autophagy, but their antibacterial activity was unknown. Using advanced microscopy and cell-free reconstitution, we show that septins polymerize into circumferentially oriented filaments that inhibit bacterial growth. RNA-sequencing reveals that septin binding to Shigella upregulates type III secretion system (T3SS) effectors and triggers a stress response. Underlying this, septin-bound Shigella possess hyperpolarized membranes, but without lethally damaged cell envelope. We show that exposure to septin binding enhances the impact of the cell envelope-targeting antibiotic polymyxin B. During infection, T3SS effector OspC1 prevents septin recruitment, while stress-response regulator cspC protects against septin-mediated changes to membrane potential. Hence, septin binding is intrinsically antibacterial and is sensed by Shigella as a danger signal to promote mechanisms of intracellular tolerance.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Infection Biology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom",
    "Laboratory of Experimental Biophysics, School of Basic Sciences, Institute of Physics, Interfaculty Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland",
    "Institut Pasteur, Université Paris Cité, CNRS Unité Mixe de Recherche 6047, INSERM U1306, Unité de Biologie et génétique de la paroi bactérienne, Paris F-75015, France",
    "Pathogen Gene Regulation Unit, Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Científicas, Valencia 46010, Spain"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Gizem Özbaykal Güler"
    },
    {
      "affs": [
        1
      ],
      "name": "Chen Zhang",
      "orcid": "0000-0003-1468-2906"
    },
    {
      "affs": [
        0
      ],
      "name": "Dominik Brokatzky"
    },
    {
      "affs": [
        0
      ],
      "name": "Rajdeep Das",
      "orcid": "0000-0001-8127-9055"
    },
    {
      "affs": [
        2
      ],
      "name": "Richard Wheeler",
      "orcid": "0000-0002-9249-2377"
    },
    {
      "affs": [
        2
      ],
      "name": "Ivo Gomperts Boneca",
      "orcid": "0000-0001-8122-509X"
    },
    {
      "affs": [
        3
      ],
      "name": "Teresa Cortes",
      "orcid": "0000-0002-5746-3300"
    },
    {
      "affs": [
        1
      ],
      "name": "Suliana Manley",
      "orcid": "0000-0002-4755-4778"
    },
    {
      "affs": [
        0
      ],
      "name": "Serge Mostowy",
      "orcid": "0000-0002-7286-6503"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5n390e731x3sc.bib",
  "cited_by": 0,
  "doi": "10.1073/pnas.2529720123",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13732209/",
  "id": "5n390e731x3sc",
  "issns": [
    "0027-8424",
    "1091-6490"
  ],
  "landing_url": "https://doi.org/10.1073/pnas.2529720123",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "423"
    ],
    [
      "heading",
      "Biological Sciences"
    ],
    [
      "heading",
      "Microbiology"
    ],
    [
      "openalex",
      "2404|Escherichia coli research studies"
    ]
  ],
  "openalex": "W7223985317",
  "pdf_url": "https://www.pnas.org/doi/pdf/10.1073/pnas.2529720123",
  "pmcid": "PMC13732209",
  "pmid": "42945259",
  "published": "2026-10-27",
  "publisher": "National Academy of Sciences",
  "ris": "https://research.draconitic.com/paper/5n390e731x3sc.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:22:14Z",
      "native_id": "PMC13732209",
      "source": "pmc",
      "url": "https://doi.org/10.1073/pnas.2529720123"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "heading:Microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Septin binding restricts Shigella growth and triggers intracellular tolerance",
  "title_text": "Septin binding restricts Shigella growth and triggers intracellular tolerance",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:22:14Z",
  "url": "https://research.draconitic.com/paper/5n390e731x3sc",
  "venue": "Proceedings of the National Academy of Sciences of the United States of America",
  "versions": []
}