{
  "abstract": "Objective: To investigate the effects of Lycium barbarum polysaccharides (LBPs) on the methylation of the Suppressor of Cytokine Signalling-1 (SOCS-1) gene and the maturation and migration of human monocyte-derived dendritic cells (DCs) in vitro .\n\nMethods: Human monocyte-derived DCs were generated using recombinant human GM-CSF and interleukin-4 (IL-4) and treated with various concentrations of LBPs (50, 100, and 200 mg/L). SOCS-1 methylation and relative gene expression were analyzed by methylation-specific quantitative PCR (qMSP) and quantitative real-time PCR, respectively. DC surface markers were assessed by flow cytometry. Cytokine (IL-12 and IL-10) secretion was measured by enzyme-linked immunosorbent assay (ELISA). JAK2, STAT3 and STAT5 phosphorylation were assessed by Western Blot Analysis. The ability of LBPs-treated DCs to stimulate lymphocyte proliferation was evaluated in a mixed lymphocyte reaction (MLR). DC migration was tested using a Transwell assay toward MIP-3β, and CCR7 expression was quantified by real-time PCR.\n\nResults: LBPs treatment upregulated SOCS-1 gene methylation and increased the expression of maturation markers (CD80, CD83, HLA-DR) and IL-12 production in a dose-dependent manner. Conversely, SOCS-1 expression and IL-10 secretion were downregulated. Furthermore, LBPs-treated DCs enhanced allogeneic lymphocyte proliferation. A significant increase in DC migration toward MIP-3β and CCR7 expression was observed in the 200 mg/L LBPs group compared to the control.\n\nConclusion: These findings indicate that LBPs are associated with increased SOCS-1 methylation and enhanced maturation and migratory capacity of human monocyte-derived DCs in vitro . Notably, to our knowledge, this is the first report demonstrating that a plant-derived polysaccharide can modulate SOCS-1 gene methylation, suggesting a potential epigenetic mechanism underlying the immunomodulatory activity of LBPs.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Education and Research, Changzhou Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine , ,",
    "Department of General Medicine, Health Center of Nanjing Audit University , ,",
    "Department of Hepatobiliary and Pancreatic Surgery, Peking University Shenzhen Hospital , ,",
    "Department of Clinical Laboratory, Changzhou West Taihu Hospital , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Wei Pan"
    },
    {
      "affs": [
        0
      ],
      "name": "Lijia Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Yin Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Yuanjun Jiao"
    },
    {
      "affs": [
        0
      ],
      "name": "Huili Gu"
    },
    {
      "affs": [
        3
      ],
      "name": "Jiayan Gao"
    },
    {
      "affs": [
        3
      ],
      "name": "Lixin Jiang"
    },
    {
      "affs": [
        3
      ],
      "name": "Gaochao Qian"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5npw1wagzhnq8.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1921722",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668845/?tool=EBI",
  "id": "5npw1wagzhnq8",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13668845",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2403|Immunotherapy and Immune Responses"
    ]
  ],
  "openalex": "W7215113026",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668845/pdf/?tool=EBI",
  "pmcid": "PMC13668845",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/5npw1wagzhnq8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:18Z",
      "native_id": "PMC:PMC13668845",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13668845"
    },
    {
      "first_seen": "2026-10-30T12:19:15Z",
      "native_id": "10.3389/fimmu.2026.1921722",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1921722/full"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Immunotherapy and Immune Responses",
      "subfield": "immunology"
    }
  ],
  "title": "Lycium barbarum polysaccharides modulate SOCS-1 gene methylation and promote the maturation and migration of human monocyte-derived dendritic cells in vitro",
  "title_text": "Lycium barbarum polysaccharides modulate SOCS-1 gene methylation and promote the maturation and migration of human monocyte-derived dendritic cells in vitro",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:15Z",
  "url": "https://research.draconitic.com/paper/5npw1wagzhnq8",
  "venue": "Frontiers in immunology",
  "versions": []
}