{
  "abstract": "Background: Erythrocyte proteins released into plasma carry oxidation-related annotations and can be read as evidence of oxidative stress. We examined whether this applies to an oxidative-stress enrichment reported in a plasma proteomic study of obstructive sleep apnoea (OSA). Methods: In a secondary analysis of public data (PXD073409; men; 15 non-OSA, 15 mild OSA, 15 severe OSA; 422 proteins), erythrocyte, platelet and coagulation quality marker indices were computed from the panels of Geyer et al.; the erythrocyte panel was only partly quantified (15 of 29 entries). The original differential and over-representation analyses were repeated with panel members excluded, with adjustment for the erythrocyte index, and with leave-one-protein-out indices. A second public dataset (PXD069503; 15 severe OSA, 15 mild-to-moderate OSA, 15 controls) was used for directional comparison. Clinical covariates were not available. Results: The erythrocyte index was higher in mild (ratio of geometric means 1.74, 95% CI 1.04 to 2.80) and severe OSA (2.43, 1.68 to 3.47) than in non-OSA participants. Six of the 13 proteins that are increased in severe OSA were erythrocyte panel members, and all nine enriched terms consisted of panel members only, including hydrogen peroxide catabolic process (HBA1, HBB, HBD, PRDX2). After adjustment, PRDX2 was accounted for by erythrocyte content, whereas the haemoglobin chains remained elevated and carried seven of the terms. The index alone separated severe- from non-OSA participants (AUROC 0.858); the data could not show whether all 422 proteins (AUROC 0.969) add to it (difference +0.111, 95% CI −0.009 to +0.268). In the second dataset the index was higher in severe OSA than in controls (1.55, 1.06 to 2.35). Conclusions: The reported oxidative-stress enrichment rests on erythrocyte-associated proteins and, on these data, is not evidence of altered oxidative activity in plasma. Whether this content arises before or after venepuncture cannot be determined. Quality marker indices should be reported alongside plasma proteomic biomarker results.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, Malatya 44280, Türkiye",
    "Department of Biostatistics, and Medical Informatics, Faculty of Medicine, Balikesir University, Balikesir 10145, Türkiye",
    "Department of Therapy and Rehabilitation, Kozaklı Vocational School, Nevşehir Hacı Bektaş Veli University, Nevşehir 50600, Türkiye",
    "Department of Computer Sciences, College of Computer and Information Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia",
    "Department of Physiology, College of Medicine, King Khalid University, Abha 61421, Saudi Arabia",
    "Department of Cariology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, 41390 Gothenburg, Sweden",
    "Department of Applied Dental Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan",
    "Department of Pathology, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai 602 105, Tamil Nadu, India"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Seyma Yasar",
      "orcid": "0000-0003-1300-3393"
    },
    {
      "affs": [
        1
      ],
      "name": "Fatma Hilal Yagin",
      "orcid": "0000-0002-9848-7958"
    },
    {
      "affs": [
        2
      ],
      "name": "Busra Nur Findik",
      "orcid": "0000-0002-1811-3164"
    },
    {
      "affs": [
        3
      ],
      "name": "Sarah A. Alzakari"
    },
    {
      "affs": [
        3
      ],
      "name": "Amal K. Alkhalifa",
      "orcid": "0000-0002-7273-4041"
    },
    {
      "affs": [
        4
      ],
      "name": "Fahaid Al-Hashem",
      "orcid": "0000-0001-5795-9966"
    },
    {
      "affs": [
        5,
        6,
        7
      ],
      "name": "Abedelmalek Kalefh Tabnjh",
      "orcid": "0000-0001-8263-4944"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/5p5k90penkan8.bib",
  "cited_by": 0,
  "doi": "10.3390/jcm15208081",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705657/?tool=EBI",
  "id": "5p5k90penkan8",
  "issns": [
    "2077-0383"
  ],
  "landing_url": "https://www.mdpi.com/2077-0383/15/20/8081",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2737|Obstructive Sleep Apnea Research"
    ]
  ],
  "openalex": "W7222929803",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705657/pdf/?tool=EBI",
  "pmcid": "PMC13705657",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/5p5k90penkan8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:01Z",
      "native_id": "10.3390/jcm15208081",
      "source": "crossref",
      "url": "https://www.mdpi.com/2077-0383/15/20/8081"
    },
    {
      "first_seen": "2026-10-30T12:21:45Z",
      "native_id": "PMC:PMC13705657",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13705657"
    }
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Obstructive Sleep Apnea Research",
      "subfield": "physiology"
    }
  ],
  "title": "Erythrocyte-Associated Proteins Underlie the Reported Oxidative-Stress Enrichment in Plasma Proteomics of Obstructive Sleep Apnoea: A Secondary Quality Marker Analysis of Public Datasets",
  "title_text": "Erythrocyte-Associated Proteins Underlie the Reported Oxidative-Stress Enrichment in Plasma Proteomics of Obstructive Sleep Apnoea: A Secondary Quality Marker Analysis of Public Datasets",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:45Z",
  "url": "https://research.draconitic.com/paper/5p5k90penkan8",
  "venue": "Journal of Clinical Medicine",
  "versions": []
}